分泌小鼠抗人甲胎蛋白单克隆抗体杂交瘤细胞株的建立

Establishment of moue hybrids secreting monoclonal antibodies to human alpha fetoprotein

采用活体内生长 SP2 / 0骨髓细胞为融合伙伴 ,用 SP2 / 0细胞与甲胎蛋白 (AFP)免疫的 BALA/ C小鼠脾细胞进行融合 ,建立了 2株稳定分泌抗 AFP单克隆抗体 (Mc AB)的小鼠杂交瘤株 (HMA2 15和HMA62 )。冻存两年后复苏 ,仍稳定地分泌特异抗体。培养上清的抗体效价达 10 -3 ,腹水效价达 10 -6 ,HMA2 15和 HMA64识别抗原表位不同 ,HMA2 15和 HMA64均属于小鼠 Ig G1亚类。结果表明两株细胞系能稳定地分泌抗人 AFP单抗 ,可为诊断和治疗的研究提供新的材料。

Two mouse hybrids(HMA215 and HMA64)secreting monoclonal antibodies(McAb)to human alpha fetoprotein(AFP)were established by fusion of SP2/0 mouse myeloma cells with spleen cells from immunized with AFP the hybrids kept secret specific antibodies after two years storage The titer of McAb in the supernatant of culture cells was 10 -3 and the ascites fluid was 10 -6 Two McAbs recognized two different epitopes and both belong to lgG1 subclass It was shown that the McAb might be a new material for diagnosis and treatment research