IL-6基因-572C／G多态性对2型糖尿病患者血清IL-6水平的影响

Influence of Interleukin-6-572C/G Polymorphism on Serum Interleukin-6 Level in Patients with Type 2 Diabetes Mellitus

【目的】探讨白介素-6（IL-6）基因-572C／G多态性对2型糖尿病患者血清IL-6水平的影响。【方法】应用聚合酶链反应-限制性片段长度多态性方法检测358例初发2型糖尿病患者IL-6基因-572C／G多态性，用ELISA方法检测不同基因型患者的IL-6水平【结果】2型糖尿病患者IL-6基因-572CC，CG，GG基因型人数分别为212，129，17；血清IL-6水平分别为（61．32±16．98）pg／mL，（64．98±15．19）pg／mL和（92．18±25．36）pg／mL，血清IL-6水平在CC，CG基因型间差异无显著性，两种基因型与GG基因型相比，血清IL-6水平差异均有显著性（P〈0．05）。【结论]IL-6基因572C／G多态性可能具有功能性，572GG基因型分泌IL-6能力可能较CC及CG基因型强，IL-6基因572GG基因型可能是2型糖尿病的危险因素之一。

[Objective]To explore the influence of interleukin 6 （IL-6）/-572C/G polymorphism on serum IL-6 level in patients with type 2 diabetes mellitus（T2DM）. [Methods]Potymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to determine the distribution of allele and genotype frequencies of IL-6 572C/G polymorphism in 358 T2DM patients. Serum IL-6 levels in patients with various genotypes were examined by ELISA. [Results]The number of carriers with IL-6 572 CC, CG and GG genotype were 211, 129 and 17, respectively, and serum IL 6 levels of them were 61.32±16.98pg/mL, 64.98±15.19 pg/mL and 92. 18±25. 36pg/mL, respectively. There was no significant difference in serum IL-6 level be tween CC and CG genotype, but there was significant difference between CC or CG genotype and GG genotype （ P 〈 0.05）. [Conclusion] IL-6-572C/G polymorphism may be functional. The secretion capacity of IL-6 in the carriers with 572GG genotype is stronger than that in the carriers with 572CC and 572CG genotype. IL-6- 572GG genotype may be one of the risk factors of T2DM.