转SNC1基因抗枯萎病棉花后代组织结构抗性分析

Resistance analysis on Anatomical Structure of Transgenic Cotton with SNC1 Gene to Resist Fusarium Wilt

【目的】棉花抵御外来病原物侵害的途径是多种多样的,研究抗病转基因棉和非转基因棉在组织结构上的抗性差异。【方法】利用茎尖遗传转化获得的转SNC1(suppressor of npr1-1,constitutive)基因棉花"中35"和"军棉1号",通过营养钵伤根法对其接种棉花枯萎病菌菌株,借助石蜡切片技术,观察棉株根茎部细胞解剖结构。【结果】抗病转基因棉根茎部胼胝质、侵填体和胶质体形成多于非转基因棉,并且韧皮部细胞排列紧密,规整,细胞间隙小。【结论】组织结构抗性在棉花抵御外来病原物侵害时所起到的至关重要的作用。

【Objective】The plant had various kinds of pathway to defend pathogenic bacterias to analyze resistance differences of the anatomical structure between the non-transgenic and the transgenic cotton.【Method】The transgenic ＂Zhong 35＂ and ＂No.1 Junmian＂ transferred SNC1（suppressor of npr1-1,constitutive） were obtained by use of genctic transformation of stem top.Then through wax section,the anatomical structure of the root and stem of cotton plant was observed.【Result】There were more callosities,tylosis and colloids in the root and stem of transgenic cotton than those of non-transgenic cotton.And the phloem cell of transgenic cotton disposed tightly and the intercellular space were small.【Conclusion】These results showed that the anatomical structure played the important role in defending pathogenic bacterias.