博格达绒山羊KAP13.1基因的多态性研究

The Polymorphism of KAP13.1 Gene in Bogeda Cashmere Goat

【目的】寻找影响毛绒性状的候选基因,探讨该基因是否能作为影响毛绒性状的候选基因之一。为后期新疆山羊地方品种的分子育种提供一定的理论依据。【方法】试验以随机采集的279个博格达绒山羊个体的血样为材料,利用PCR-RFLP、DNA测序等技术研究分析博格达绒山羊KAP13.1基因的多态性。【结果】博格达绒山羊KAP13.1基因具有多态性,KAP13.1基因存在三种基因型:TT、GT和GG,且处于Hardy-Weinberg非平衡状态(P>0.05),KAP13.1基因编码区片段在基因组c.186位置发生错义突变T>G,导致氨基酸p.48Leu>Arg的变化。【结论】KAP13.1基因可能是影响绒山羊产绒性状的候选基因之一。

【Objective】This study was conducted to look for the candidate gene which affect cashmere traits to provide certain theoretical basis for molecular breeding in Xinjiang goat local breeds.【Method】In this experiment,the test materials are Bogeda Cashmere goat individual（n=279） of blood samples with random collection,the polymorphism of KAP13.1 gene in Bogeda Cashmere goat was detected by PCR-RFLP and DNA sequencing methods and so on,to explore the gene which whether is probably one of the candidate genes affecting cashmere traits.【Result】The results showed,KAP13.1 gene in Bogeda Cashmere goat had polymorphism,in the KAP13.1 gene exist three kinds of genotype： TT,GT and GG,and the genotype distribution was in disagreement with Hardy-Weinberg equilibrium（P0.05）,there was a missense mutation（c.186 T G）,it is leads to p.48 Leu Arg.【Conclusion】This shows that KAP13.1 gene is probably one of the candidate genes with affecting cashmere traits.