摘要
目的建立新型LC/LC-UV系统测定血浆中葛根素浓度的方法,用于葛根素人体或动物的药物动力学研究。方法 LC1色谱柱FRO-XB C18(30 mm×4.6 mm,20μm,ANAX),流动相为20 mmol.L-1醋酸铵溶液-乙腈=75∶25(v/v),流速为1.0 mL.min-1,中央处理体系温度为40℃,LC2系统中分析色谱柱为AC-XB C18(300mm×4.6 mm,5μm,ANAX),流动相为20 mmol.L-1醋酸铵溶液(用甲酸调pH到3.8)-乙腈=86∶14(v/v),流速为1.2 mL.min-1,检测波长为248 nm。目标物从LC1转移到LC2,聚焦流速为1.0 mL.min-1,目标物转移窗口为1.66～2.20 min;定量环为200μL,样品用15%三氯醋酸预处理。结果葛根素在0.020～4.50μg.mL-1与峰面积线性关系良好,平均相关系数为0.999 4,最低定量浓度为0.015μg.mL-1。日内RSD<3.0%(n=6),日间RSD<5.0%(n=18),准确度在100.3%～102.6%。结论新型LC/LC-UV系统的葛根素测定方法操作步骤少、准确性与精密度良好、自动化程度高,适合于葛根素血药浓度测定及动力学研究。
Objective To establish a new LC/LC-UV method for the determination of puerarin and for pharmacokinetics of puerarin.Methods LC1 system column was FRO-XB C18（30 mm×4.6 mm,20 μm,ANAX）,with the mobile phase consisting of 20 mmol·L-1 ammonium acetate-acctonitrile（75∶25,v/v） at 1.0 mL·min-1.The column temperature was 40 ℃.LC2 system column was UC-XB C18（300 mm×4.6 mm,5 μm,ANAX）,with the mobile phase consisting of 20 mmol·L-1 ammonium acetate（pH 3.8 adjustted by formic acid）：acetonitrile（86∶14,v/v） at 1.2 mL·min-1.The detection wavelength was 248 nm.The flow rate was 1.0 mL·min-1,and the window of ＂heart cutting＂was 1.66-2.20 min.The sample loop volume was 200 μL.Results The linear range of puerarin was 0.020-4.50 μg·mL-1（r=0.999 4）.The limit of puerarin was 0.015 μg·mL-1.The intra-day RSD was〈3.0%（n=6）,and the inter-day RSD was〈5.0%（n=18）.The accuracy was 100.3%-102.6%.Conclusion The LC/LC-UV method is simple and accurate,with high automatization procedure,which can be used for pharmacokinetics of puerarin.
出处
《中南药学》
CAS
2010年第11期814-817,共4页
Central South Pharmacy
