鬼箭羽提取物抑制血管生成的实验研究

Anti-angiogenic activity of extract from Winged Euonymus Twig

目的研究鬼箭羽氯仿提取物对血管生成的影响。方法采用人脐静脉内皮细胞模型(HUVEC)、大鼠动脉环模型(rat aortic rings)和鸡胚绒毛尿囊膜(CAM)模型研究鬼箭羽提取物抑制血管生成作用。结果 8μg.mL-1鬼箭羽提取物显著抑制人脐静脉内皮细胞的增殖,抑制率达到36.2%;2μg.mL-1和4μg.mL-1鬼箭羽提取物能够显著抑制大鼠动脉环新血管结构形成,抑制率分别达到56.41%和65.25%;鬼箭羽提取物20μg.个-1与40μg.个-1对鸡胚绒毛尿囊膜(CAM)血管抑制率分别达到了22.6%与31.2%。结论鬼箭羽提取物具有显著的抗血管生成活性。

Objective To study the anti-angiogenic activity of extract from Winged Euonymus Twig.Methods Human umbilical vein endothelial cells（HUVEC） model,rat aortic ring model and chicken chorioallantoic membrane（CAM） model were used to evaluate the anti-angiogenesis effect of extract from Ramulus Euonymi respectively.Results Extract from Winged Euonymus Twig inhibited the proliferation of HUVEC with inhibition ratio of 36.2% at 8 μg·mL-1.Extract from Winged Euonymus Twig could inhibit the neovascularization of rat aortic rings with inhibition ratio of 56.41% and 65.25% at 2 and 4 μg·mL-1.In the chicken CAM experiment,extract from Winged Euonymus Twig induced 22.6% and 31.2% inhibition ratio of microvessel density at 20 and 40 μg·egg-1,respectively.Conclusion Extract from Winged Euonymus Twig showes significant anti-angiogenic activities.