摘要
本研究建立了免疫PCR技术。并以此为基础检测了血清中的肿瘤抗原。肿瘤病人血清检测结果表明免疫PCR技术对肿瘤有较大诊断价值。将构建的单链抗体和链亲和素融合蛋白基因pET21-scFvCEA-STA在大肠杆菌HMS174(DE3)(plysS)中进行表达,免疫组化及蛋白印迹均提示获得了具备结合生物素和抗原分子活性的双特异融合蛋白。
This paper describes the application of an established technique termed Immune-PCR in the detection of sero-logical tumor-associated antigen among various kinds of carcinomas. The expression of the fusion protein of single chain antibody of anti-carcinoembryonic and streptavidin was concurrently reported. The results show that this new technique has a very high sensitivity of the detection of the circulating tumor associated antigen than other existing assays such as immunoradiometric assay and radioimmunoassay. It was also proven by immunoblotting and Western blot that the purified fusion protein still had the activity capability of binding the antigen and biotin molecule.
出处
《中国科学基金》
CSCD
1999年第6期328-330,共3页
Bulletin of National Natural Science Foundation of China
基金
国家自然科学基金
批准号39400159
39870787
