摘要
通过研究壬基酚对雄性黑斑蛙(Rana nigromaculata)成体的精子和精巢的影响,探讨壬基酚对黑斑蛙的生殖毒性.用不同剂量的壬基酚对雄性黑斑蛙进行处理,对黑斑蛙的精巢系数、精子数、形态结构、畸形率和精巢显微结构等分别进行研究.结果表明,与对照组相比,随着壬基酚浓度的升高,染毒组黑斑蛙的精巢系数下降,精子数减少,精子畸形率明显增大;畸形精子主要表现为其头部出现肥大、弯曲和圆形等现象;精巢显微结构发生变化,表现为生精小管萎缩,生精细胞层次减少,间质区不明显.说明壬基酚对雄性黑斑蛙生殖系统具有毒性效应.
In order to study the effect of nonylphenol on male Rana nigromaculata reproductive activities,different doses of nonylphenol（NP） were treated with male R.nigromaculata.The sperm coefficient,number,structure,abnormality rate and testis microstructures were observed.The results showed that with the NP concentration up,sperm coefficient and number decreased,and sperm abnormality rate increased significantly.Sperm abnormalities were exhibited by sperm hypertrophy,curved and round head.The histopathological examination revealed that siminiferous tubules shrunk,the hiberarchy of siminiferous cell decreased,and sertoli sections were weaker on NP-treated groups.Nonylphenol had toxic effect on male R.nigromaculata reproductive system.
出处
《生命科学研究》
CAS
CSCD
2010年第6期538-542,共5页
Life Science Research
基金
安徽省教育厅自然科学基金资助项目(KJ2010B092)
安徽省高校青年教师科研项目(2006jql214)
关键词
壬基酚
黑斑蛙
精子
生殖毒性
nonylphenol（NP）
R.nigromaculata
sperm
reproductive toxicity
