溶藻弧菌对华贵栉孔扇贝体内几种酶活性的影响

Effects of Vibrio alginolyticus on Enzymes Activities of Chlamys nobilis

对华贵栉孔扇贝(Chlamys nobilis)注射不同密度的溶藻弧菌(Vibrio alginolyticus),于注射后6,12,18,48,72h测定体内过氧化氢酶(CAT)、超氧化物岐化酶(SOD)、碱性磷酸酶(ALP)和酸性磷酸酶(ACP)的活力。结果表明:5×107cell.mL-1,5×108cell.mL-1组血淋巴中CAT活性均有升高趋势,其中5×108cell.mL-1组在18h极显著增加(P≤0.01),24h显著增加(P≤0.05)。5×108cell.mL-1组SOD活性最初表现为抑制,在12h和18h显著低于对照组(P≤0.05),24h后明显升高,5×107cell.mL-1组与对照组相比差异不明显。肝脏中ALP活性在18h与对照组相比,5×108cell.mL-1组极显著升高(P≤0.01),5×107cell.mL-1组显著升高(P≤0.05)。注射后ACP活性有明显升高的趋势,5×108cell.mL-1组在12h和18h显著高于对照组(P≤0.05),且在注射后24h5×107cell.mL-1,5×108cell.mL-1组活性均达到最高。

The activities of CAT,SOD,ALP and ACP of Chlamys nobilis were assayed at 6 h,12 h,18 h,48 h and 72 h after challenged with Vibrio alginolyticus injection. The results showed that the CAT activities in haemolymph were higher in 5×107 cell·mL-1 and 5×108 cell·mL-1 groups than that in control group. The activity of CAT in 5×108 cell·mL-1 group increased extremely significantly at 18 h（P≤0.01）and significantly at 24 h（P≤0.05）; the activity of SOD in 5×108 cell·mL-1 group were inhibited at first time and lower than that of control group at 12 h and 18 h significantly（P≤0.05）,and increased after 24 h,there was no significant difference between 5×107 cell·mL-1 group and control group; compared with that of control group at 18 h,the activities of ALP in liver in 5×108 cell·mL-1 group increased extremely significantly（P≤0.01）and significantly at 24 h（P≤0.05）; the activities of ACP in 5×108 cell·mL-1 group were increased significantly at 12 h and 18 h（P≤0.05）,and the highest activity was attained at 24 h in 5×107 cell·mL-1 and 5×108 cell·mL-1 groups.