转SNC1基因棉花抗枯萎病防御酶活性分析

Analysis on the Defense Enzyme Activity in Transgenic Cotton Transferring SNC1 Gene Inoculated with Fusarium oxysporum f. sp. Vasinfectum

以转SNC1基因的棉花中35和军棉1号和对应的非转基因棉花叶片为材料,接种棉花枯萎病病菌,以分析与主要抗病性有关的5类防御酶系。接菌后,和对照相比,转SNC1基因棉花和非转基因棉花防御酶的活性变化差异明显。其中,转基因中35的PAL、SOD、POD和PPO酶活性均高于非转基因中35,并且在接菌第2,4天达到峰值,然后随时间增加呈下降趋势,而CAT酶活性低于非转基因中35,随时间增加变化趋势不明显;转基因军棉1号的PAL、SOD、PPO和酶CAT活性变化趋势同转基因中35,而POD酶活性变化趋势却相反,低于非转基因军棉1号。通过本试验表明,以抗病品种和感病品种为受体得到的转基因棉在受到病原菌侵染后,酶活性变化有差异。其中PAL、SOD、PPO和CAT酶活性变化趋势稳定,其酶活性高低可以作为转基因棉花抗病性的生化指标,同时表明SNC1基因的抗病性在陆地棉中得到体现。

To order to compare the difference of enzyme activity of the transgenic and non-transgenic cotton.We inoculated transgenic cotton transferring SNC1 disease-resistance gene with Fusarium oxysporum f.sp.Vasinfectum.The transgenic and non-transgenic cotton changed significantly in defense enzyme activity.The PAL,SOD,POD and PPO activity of the transgenic Zhong 35 were more high than the non-transgenic Zhong 35′s,and up to the peak at 2-4 day.And the CAT activity was more low than the non-transgenic Zhong 35′s;The changed trend of the PAL,SOD,PPO and CAT activity of the transgenic No.1 Junmian were as same as the transgenic Zhong 35′s.However,the POD activity of the transgenic No.1 Junmian was more low than the non-transgenic No.1 Junmian′s,and was opposite to the transgenic Zhong 35′s.The results showed that the PAL,SOD,PPO and CAT activity of the transgenic cotton were used as the biochemistry index,and showed that SNC1 gene played a important role at disease resistance.