植物分子系统发育与进化研究的基因选择、引物设计与应用策略

Gene selection,primer design and their utility on the studies of plant molecular phylogeny and evolution

引物选择、设计与应用策略是植物分子系统发育与进化研究的关键环节。本文综述了基因选择的原则、引物设计的技巧以及如何有效地利用所涉及的片段获取相应的PCR片段的方法。

Primer selection, design and utility are key and absolutely necessary steps on the studies of plant molecular phylogeny and evolution. Here the principles of gene selection, the skills of primer design and the efficient utility of the primers selected to obtain the needed PCR products are summarized.