基于SRAP分析苎麻及近缘种的系统学关系

Phylogenetic relationship of ramie and its wild relatives based on SRAP markers

苎麻与近缘种的系统学关系一直存在争议。利用26对多态性好的随机引物,对中国苎麻属的17种9变种的共27份材料进行SRAP分子标记分析。扩增结果是26对引物共扩出368条带,其中363条为多态性带,占98.64%。聚类分析表明:(1)腋球苎麻组的腋球苎麻和苎麻组的苎麻、贴毛苎麻聚在一支并靠近系统树的根部,支持王文采苎麻组起源于原始的腋球苎麻组观点。(2)苎麻和贴毛苎麻聚在一支,支持郭安平苎麻直接起源于贴毛苎麻的观点。(3)苎麻组的青叶苎麻、微绿苎麻和大叶苎麻组的多倍体类群聚在一支,可能是由于在种间杂交导致。

It was considered that there was disputation on the systematics of the genus Boehmeria,as well as the relationship among different sections in this genus. The phylogenetic relationship of 17 species and 9 varieties of Boehmeria was investigated using SRAP analysis based on a total of 27 samples. 368 bands were produced by SRAP technique using 26 primer pairs,of which 363（98.64%）bands were polymorphic.It was also indicated by clustering analysis that：（1）Boehmeria malabarica var.malabarica（sect.Boehmeria）,B.nivea（sect.Tilocnide）and B.nivea var.nipononivea（sect.Tilocnide）were clustered into one branch and near the root of the phylogenetic tree,indicating that sect.Tilocnide originated from sect.Boehmeria;（2）B.nivea and B.nivea var.nipononivea were clustered into one branch,indicating that B.nivea originated directly from B.nivea var.nipononivea;（3）B.nivea var.viridula（sect.Tilocnide）and B.nivea var.tenacissima（sect.Tilocnide）were clustered together with the polyploid of sect.Duretia,which might have resulted from interspecific hybridization.