胃癌组织中β-catenin不同表达类型的临床意义及其对预后的影响

The clinical and prognostic significance of the different β-catenin expression types in gastric cancer after surgery

目的:研究β-catenin在胃癌中不同表达类型的临床意义及与预后的关系。方法:采用免疫组化ABC法结合组织芯片技术,检测90例胃癌组织中β-catenin蛋白表达。结合临床资料和随访资料进行统计学分析,探讨不同β-catenin表达类型的临床特征以及与胃癌预后的关系。结果:β-catenin胞浆和核表达18例,膜表达缺失40例,膜表达32例。β-catenin阳性(胞浆、核累积和膜表达缺失)与肿瘤浸润深度、淋巴结转移以及肿瘤TNM分期相关(P均<0.01)。β-catenin阳性表达胃癌患者术后1、3、5年生存率分别为67.2%、27.6%、17.2%,明显低于阴性表达组的90.6%、78.1%、61.3%,差异具有统计学意义(P<0.001);Cox风险比例模型分析显示β-catenin阳性表达是影响术后生存的独立预后因素(P=0.002)。结论:β-catenin的异常表达与胃癌侵袭、转移密切相关,可以用来预测胃癌患者术后的预后。

Objective： To study the clinical and prognostic significance of the different β-catenin expression types in gastric cancer.Methods： The expressions of β-catenin protein in 90 gastric cancer tissues were detected in 90 gastric cancer tissues by immunohistochemistry on tissue microarray.Results： There were 18 cases with β-catenin cytoplasm and nuclear accumulation,40 cases with membrane expression absence,and 32 cases with membrane expression.The positive for β-catenin expression was defined as cytoplasm and nuclear accumulation and membrane expression absence.The expression of β-catenin was related to the depth of invasion,metastasis of lymph nodes and TNM stage（P〈0.01）.The univariate and multivariate analyses revealed that β-catenin was a significant predictor for overall survival（P〈0.001 and P=0.002,respectively）.The 1-,3-,5-year overall survival rates of the β-catenin（-） group were significantly higher than those of the β-catenin（＋） group（90.6%,78.1% and 61.3% vs.67.2% and 27.6%,17.2%,respectively）.Conclusion： The aberrant expression of β-catenin in gastric cancer was associated with high invasiveness and metastatic potential.It can be used to predict the prognosis of patients with gastric cancer after surgery.