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RAPD标记鉴别红鳍东方鲀和假睛东方鲀种群的初步研究 被引量:9

IDENTIFICATION OF THREE POPULATIONS (CHINESE FUGU RUBRIPES, JAPANESE FUGU RUBRIPES AND FUGU PSEUDOMMUS) USING RAPD MARKERS
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摘要 用RAPD技术对东方纯属(Fugu)的红鳍东方鲀(Fugu rubripes)和假睛东方鲀(Fugupseudommus)共三个群体进行分析,经20个随机引物扩增,得到每一个体的多态片段。比较所有图谱,没有发现能够鉴别三个群体的特异性谱带;而且采自日本的红鳍东方纯与采自中国的红鳍东方鲀的电泳图谱基本相似。结果还表明,红鳍东方纯与假睛东方鲀电泳图谱的差异也甚小,说明二者的亲缘关系非常接近,这与以前用同工酶进行分析的结果相一致(王可玲等,1984), Three populations including Fugu rubripes domesticated in China, Fagu rubripes domesticated in Japan and natural Fugu pseudommus in Qingdao were analyzed using the RAPD technology and the pattern of each sample was obtained. Altogether 20 primers were used in this experiment. No markers were found to distinguish them in the study and the patterns of Fugu rubripes from Japan and Fugu rubripes from China are almost the same. At the same time, the difference of the patterns between Fugu rubripes and Fugu pseudommus is very small. It shows that the affinities between them are very near.
出处 《海洋湖沼通报》 CSCD 北大核心 1999年第4期38-42,共5页 Transactions of Oceanology and Limnology
基金 "948"办公室红鳍东方鲀纯种引进项目
关键词 RAPD标记 鉴别 红鳍东方TUN 假晴东方Tun 种群 RAPD marker, population identification, Fugu rubripes , Fugu pseudommus
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  • 1何舜平,汪亚平,陈宜瑜.五种鲤科鱼类的RAPD分析兼论稀有鮈鲫的系统位置[J].水生生物学报,1997,21(3):262-267. 被引量:47
  • 2成庆泰.中国东方Tun属鱼类分类研究[J].动物学报,1975,21(4):395-378.
  • 3王可玲.东方Tun属鱼类肌浆蛋白的种间差异及其聚类的分析[J].海洋与湖沼,1984,15(5):493-499.
  • 4王可玲,海洋与湖沼,1984年,15卷,5期,493页
  • 5成庆泰,动物学报,1975年,21卷,4期,395页

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