闽产厚朴叶中厚朴酚、和厚朴酚提取工艺研究

Study on Extraction Process of Magnolol and Honokiol in the Leaves of Magnolia officinalis Rehd. et Wils in Fujian

目的探讨闽产厚朴叶厚朴酚及和厚朴酚的提取工艺及其含量测定方法。方法采用正交设计实验,以厚朴叶中的厚朴酚及和厚朴酚含量为考察指标,对影响厚朴叶厚朴酚及和厚朴酚提取率的因素进行探讨,采用HPLC法测定其含量。结果超声波提取法对厚朴叶的厚朴酚及和厚朴酚提取率最高;最佳工艺为:甲醇为溶剂,料液比1∶75(g/mL),50℃,超声20 min。并用HPLC建立了其含量测定方法,厚朴酚在10～60μg.mL-1范围内线性良好,r=0.9996;和厚朴酚在20～120μg.mL-1范围内线性良好,r=0.9997,含量分别为0.09%和0.16%,两酚平均含量为0.25%。结论采用超声波提取法提取,HPLC进行含量测定,操作简便,稳定性好,结果准确,适合用于厚朴叶厚朴酚及和厚朴酚的提取和含量测定。

Objective To explore the optimal extraction process and determination method of magnolol and honokiol in leaves of Magnolia officinalis Rehd.et Wils in Fujian.Methods The orthogonal design was used to optimize the extraction process.The influential factors of extraction rate were analyzed.The contents of magnolol and honokiol were taken as the index for investigation and determined by HPLC.Results The highest extraction rate could be obtained with the ultrasonic extraction method,and the optimal conditions for the extraction by using methanol as solvent were obtained as follows the ratio of material to solvent 1：75 at 50℃ for 20 min.The linear range of magnolol was 10-60 μg.mL-1（r=0.9996）.The linear range of honokiol was 20-120 μg.mL-1（r=0.9997）.The content of magnolol was 0.09%,honokiol was 0.16% and the average content of total phenol was 0.25%.Conclusion The method is simple,stable and accurate,and suitable for the extraction and determination of magnolol and honokiol in leaves of Magnolia officinalis Rehd.et Wils.