人参皂甙-Rh_2对人乳腺癌MCF7/AdrR细胞增殖和凋亡的影响

Efficacy of Gensenoside Rh_2 on proliferation and apoptosis for MCF7/AdrR human breast cell line

目的研究人参皂甙-Rh2(GS-Rh2)对乳腺癌MCF7/AdrR细胞增殖和凋亡的影响。方法以MCF7/AdrR细胞不加药物干预,作为对照组;以Rh2单独作用于MCF7/AdrR细胞,作为Rh2组;以低浓度阿霉素(ADM)单独作用于MCF7/AdrR细胞,作为ADM组;以Rh2和阿霉素联用于MCF7/AdrR细胞,作为Rh2+ADM组。用MTT比色法观察GS-Rh2对体外培养的MCF7/AdrR细胞生长的抑制作用;用荧光显微镜观察用药前后MCF7/AdrR细胞的形态学变化;用流式细胞仪检测凋亡细胞和细胞周期变化;用Westernblot的方法检测Fas、Bax、Bcl-2及Bcl-xL蛋白表达的变化。结果 MCF7/AdrR细胞经GS-Rh2作用后,生长受抑制,呈剂量和时间依赖性;显微镜显示,经GS-Rh2作用后,MCF7/AdrR细胞呈较明显的凋亡形态学改变,GS-Rh2能将细胞周期阻滞于G0/G1期,同时可能参与促凋亡蛋白Fas、Bax表达升高及抑制凋亡蛋白Bcl-2表达降低;但在GS-Rh2处理前后Bcl-xL无明显变化。结论 GS-Rh2能抑制体外培养的MCF7/AdrR细胞增殖并诱导其凋亡,且这种作用可能是通过上调Fas、Bax表达、下调Bcl-2表达以及阻滞细胞周期而实现。

Objective To examine the effect of Gensenoside Rh2（GS-Rh2）on proliferation and apoptosis MCF7/AdrR human breast cell line.Methods MCF7/AdrR cells were divided into control group,added with normal saline;ADM group,added with adriamycin（ADM）at the low concentration;Rh2 group,added with Rh2;and ADM ＋ Rh2group,added with ADM at the low concentration and Rh2.Cell growth inhibition was evaluated by MTT colorimetric assay.Cellular morphology was observed under microscope.Apoptotic cells and the changes of cell cycle were determined by flow cytometry.Western blot was used to detect the protein expression of Fas,Bax,Bcl-2 and Bcl-xL.Results The inhibition of GS-Rh2 on the growth of MCF7/AdrR cells was in a time-and dose-dependent manner by MTT.GS-Rh2 was capable of arresting the cell cycle at G0/G1 phase.Pro-apoptotic factors,Fas and Bax were remarkably higher than control cells,while the anti-apoptotic factors,Bcl-2 were significantly lower than control cells,but the expression of Bcl-xL protein did not change significantly.Conclusion GS-Rh2 exhibited growth inhibition effect and induced apoptosis on MCF7/AdrR cell line in vitro.Apoptosis of MCF7/AdrR cells induced by GS-Rh2 maybe related with arresting cell cycle,up-regulating Fas,Bax protein expression and down-regulating mutated Bcl-2 protein expression.