^(60)Co-γ射线辐照毛竹种子的过氧化物同工酶和RAPD分子检测的研究

Research of the POD Isoenzyme and RAPD Molecular Detection on MOSO by ^(60)Co-γIrradiation

利用6种不同剂量的^(60)Co-γ射线辐照毛竹种子,对辐照后的毛竹种子进行了过氧化物酶(POD)同工酶检测和RAPD分子检测。结果表明,过氧化物酶的活性在30 Gy和120 Gy时明显增强,其原因是毛竹在^(60)Co-γ射线辐照胁迫下的保护效应和伤害效应。RAPD分析表明毛竹不同辐照剂量之间的DNA存在明显差异,高剂量γ射线辐照对DNA分子的影响较大,能引起碱基序列的改变;对碱基序列差异片段测序并比对,发现部分片段与已知序列有高达83%的同源性,部分差异片段与GeneBank中的已知序列没有较高的同源性,应该属辐照引起的DNA序列变异。同工酶和RAPD分子检测为毛竹的诱变育种参数的制定提供了理论依据。

Moso seeds were irradiated by 60 Co-γ ray with six different dosages. The POD isoenzyme and RAPD were tested on irradiated Moso seeds. The results indicated that the POD isoenzyme activity enhanced markedly at 30 Gy and 120 Gy. The reason is the protective effect and harmful effect of Moso under 60Co-γ irradiation menace. The results of RAPD indicated that DNA was changed greatly with different irradiation dosages on Moso, and DNA was affected greatly by γ-ray irradiation with high dosages, and sequences of base pair were changed ; Differential fragments were sequenced and compared. The results showed that some fragments had higher homeology with the known sequence and the homeology was 83 %, and some fragments did not have higher homeology with the sequence of GeneBank and this could account for the sequence of DNA changed by γ-ray irradiation. The tests of POD isoenzyme and RAPD could supply theoretical evidence for the parameter formulation of Moso＇ s mutation breeding.