儿童乙型病毒性肝炎病毒携带者血清新蝶呤水平分析

Detection of serum neopterin in hepatitis B virus Carrier among children

目的研究儿童乙型病毒性肝炎病毒(HBV)携带者血清新蝶呤水平变化及其与HBV复制的关系。方法采用酶联免疫吸附试验(ELISA)法和实时荧光定量PCR法分别检测150例儿童HBV携带者和60名健康儿童的血清新蝶呤水平、HBV DNA载量。结果慢性HBV携带者血清新蝶呤水平明显高于非活动性HBsAg携带者和健康儿童,差异有统计学意义(均P<0.05);非活动性HBsAg携带者和健康儿童的血清新蝶呤水平差异无统计学意义(P>0.05);血清新蝶呤水平与HBV DNA载量在统计学上无显著相关性(r=0.131,P>0.05)。结论在儿童HBV携带者中,慢性HBV携带者比非活动性HBsAg携带者体内更容易引起细胞免疫激活,慢性HBV携带者在随访监测中更应被关注,其血清新蝶呤水平升高能提示病毒复制,但不反映病毒复制活跃程度。

Objective To detect serum neopterin level in hepatitis B virus carrier among children and explore the relation between serum neopterin level and HBV DNA load.Methods The serum neopterin and HBV DNA load were detected for 150 children who were hepatitis B virus carriers and 60 healthy children by using enzyme linked immunosorbent assay（ELISA） and real-time fluorescence PCR.Results Compared with healthy children and inactive HBsAg carriers,the serum neopterin level was significantly higher in children with chronic HBV infection,the difference was statistical significant（P〈0.05）,the difference on serum neopterin level between inactive HBsAg carrier and healthy children had no statistical significance（P〉0.05）.There was no significant correlation between neopterin level and HBV DNA load（r=0.131,P〉0.05）.Conclusion Compared with inactive HBsAg carrier,immune cells were more easily activated in children with chronic HBV infection,more attention should be paid to chronic HBV carrier during the follow-up.The increase of neopterin level could indicate the presence of virus replication,but not the quantity of the HBV DNA load.