喹硫平对小鼠脑缺血后胶质增生及IL-1β表达作用的实验研究

Experimental research of quetiapine's effects on gliosis and IL-1β expression after cerebral ischemia

目的研究喹硫平对小鼠脑缺血后胶质增生及IL-1β表达的作用。方法实验分为4组:喹硫平加缺血组、药物对照组、缺血组、药物保护组;采用免疫组化法及激光共聚焦显微镜方法检测喹硫平对小鼠脑缺血后胶质增生及IL-1β表达的作用。结果脑缺血再灌后2h小胶质细胞激活,脑缺血再灌后2d和4d小胶质细胞和星形胶质细胞激活;脑缺血再灌后8d、2w和4w喹硫平减少GFAP表达水平。缺血组与假手术组相比较GFAP-IL-1β共表达阳性细胞数量明显增加(P<0.01);药物保护组与缺血组比较GFAP-IL-1β共表达细胞数量明显减少(P<0.01)。结论喹硫平能够减轻小鼠脑缺血后胶质增生同时降低IL-1β表达水平。

Objective To study the effects of quetiapine on gliosis and IL-1β expression after cerebral ischemia.Methods Experimental animals were divided into following four groups：vehicle＋sham surgery（Veh-Sham）,quetiapine＋sham surgery（Que-Sham）,Vehicle＋ischemia（Veh-Isc）,and quetiapine＋ischemia（Que-Isc） group.The effects of quetiapine on gliosis and IL-1β expression after cerebral ischemia were investigated through immunohistochemical technique and confocal microscope measurement.Results Microcytes were activated at 2h after cerebral ischemia-reperfusion.Both microcytes and astrocytes were activated on 2d and 4d after cerebral ischemia-reperfusion.Quetiapine showed effects to decrease GFAP level on 8d,2w and 4w after cerebral ischemia-reperfusion.GFAP-IL-1β positive cells were significantly increased in ischemia group compared with sham group（P〈0.01）.GFAP-IL-1β positive cells were significantly decreased in que＋isc group compared with ischemia group（P〈0.01）.Conclusion Quetiapine attenuated gliosis and IL-1β expression after cerebral ischemia.