摘要
对鹅副黏病毒61/GO/GD/05分离株进行主要的生物学特性试验。结果显示,该毒株的鸡胚平均死亡时间(MDT)为78 h,脑内接种指数(ICPI)为1.70;静脉接种指数(IVPI)为2.34;对鸡胚的半数感染量(EID50)为10-7.43/0.2 mL;对鹅的半数致死量(LD50)为10-1.93/0.5 mL;致病性试验表明,该毒株对21日龄非免疫鸡和16日龄非免疫鹅都具有较强的致病性。综合各项生物学特性指标,确定该毒株为强毒力GPMV毒株。对其F基因进行分子生物学分析,表明该毒株为基因Ⅶ型。
A virus named as 61/GO/GD/05 of Goose Paramyxovirus isolated from Guangdong was studied for its biological characteristics.The results showed as follows： the mean death time（MDT） of embryo eggs was 78 h,the intracerebral pathogenicity index（ICPI） was 1.70,and the intravenous pathogenicity index（IVPI） was 2.34.The 50% goose dose lethal media（LD50） was 10-1.93/0.2 mL.Pathogenicity study showed that this strain was pathogenic to both 21-day-old chickens and 16-day-old geese.All the indexes indicated that strain 61/GO/GD/05 was a virulent virus.The molecular biology analysis of F gene showed that strain 61/GO/GD/05 belonged to genotype VII.
出处
《中国兽医杂志》
CAS
北大核心
2010年第12期13-16,共4页
Chinese Journal of Veterinary Medicine
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)
广东省科技攻关(5500-E08020
2007A020100006)
公益性行业(农业)科研专项经费(200803020-08)
关键词
鹅副黏病毒
F基因
生物学特性
goose paramyxovirus
F gene
biological characteristics
