实验性脾虚大鼠小肠结构与免疫功能的变化

Studies on the changes of the small intestine histology and immune function in experimental spleen deficiency rats

本试验采用利血平复制大鼠脾虚模型,观测和探讨其小肠结构与免疫功能变化。与对照组比较结果显示,脾虚组大鼠体重显著下降(P<0.05),十二指肠、空肠和回肠的组织结构均有不同程度的损伤,绒毛长度与肠黏膜厚度均显著降低;黏膜上皮内淋巴细胞和杯状细胞数量均显著增加;绒毛顶端与黏膜下层TLR2表达量均显著降低。表明,小肠黏膜结构损伤是脾虚动物体重下降的原因之一;局部过强的免疫反应是造成小肠黏膜结构损伤的主要机制,且此过程并非通过TLR2介导;小肠结构损伤及TLR2表达的显著下降均提示其抵御病原侵袭的功能下降。

The study observed and explored the structure and immune function in the small intestine of the experimental Spleen Deficiency Syndrome（SDS） in rats that was induced by reserpine.Compared with control group,the weight of the SDS group significantly decreased（P0.05）.The structure of duodenum,jejunum and ileum were damaged on different levels of the SDS group： the villus length and mucosa thickness decreased significantly;the number of intraepithelial lymphocytes and the goblet cells predominant increased;the expression of TLR2 of the top of villus and the submucosa were all significantly decreased.The results indicate that SDS induced damage in the structure of intestinal mucosa,which may result in the weight loss of animals,and the over-immunoreactions parts may be the mechanism of the structural damage of intestinal mucosa,and TLR2 did not participate in this process.The structural damage of small intestine and the decrease of the TLR2 expression indicated that the resistance against pathogenic bacteria has decreased.