他克莫司在大鼠肝脏保存中的作用

THE PROTECTIVE EFFECTS OF TACROLIMUS IN LIVER COLD PRESERVATION

目的了解他克莫司对肝脏缺血再灌注损伤的保护作用。方法在供肝保存期间向保存液中加入他克莫司,检测保存12 h后的肝脏能量物质负荷、肝移植术后受体大鼠血清中的肝酶浓度以及胆汁分泌情况。结果保存液中加入他克莫司后,肝组织在保存期间的能量物质消耗明显小于对照组,移植术后受体大鼠血清中ALT和LDH含量明显较对照组为低,胆汁分泌量则明显高于对照组,差异均具有显著性(P<0.05)。结论他克莫司用于肝脏保存能够有效地减轻缺血再灌注损伤,提高肝细胞能荷,改善移植术后的肝脏功能。

Objective To investigate the effects of tacrolimus as a liver cold storage solution on ischemia/reperfusion injury.Methods Tacrolimus was added to liver cold storage solution,and after 12 h preservation,the contents of ATP,ADP,AMP within liver cells were observed.ALT and LDH in the recipient sera and bile production were also examined after a transplantation procedure.Results Energy consumption of liver cells preserved by tacrolimus was much lower than controls.ALT and LDH in sera of recipients who received tacrolimus-preserved livers were lower than controls,and bile productions of tacrolimus-preserved livers were much higher than controls after transplantation.Conclusions Tacrolimus as a liver storage solution can ameliorate ischemia/reperfusion injury and improve hepatic function after liver transplantation.