南方红豆衫细胞体系建立的优化

The Optimization of Building Cell Line of Taxus chinensis var.mairei

[目的]优化南方红豆杉愈伤组织诱导及训化条件。[方法]以南方红豆杉幼芽、幼茎以及幼叶为外植体,比较不同外植体诱导愈伤组织的效率,并对愈伤组织的增殖条件及悬浮细胞培养条件进行了研究。[结果]以幼芽作为外植体的诱导效果最佳;南方红豆杉外植体消毒的最佳方法为链霉素浸泡2 h+洗洁精浸泡3 h+75%酒精消毒30 s+10%次氯酸钠浸泡25 min+1‰氯化汞10 min;愈伤继代的最佳配方为B5+4.0 mg/LNAA+0.5 mg/L2.4-D+0.2 mg/LGA+0.5 mg/L6-BA+2 g/L活性碳。[结论]该研究建立了南方红豆杉的高效再生体系。

[Objective]The paper aims to optimize the abduction and domestication conditions of Taxus chinensis var.Mairei callus.[Method]We compared the efficiencies of callus between different explants and investigated the multiplication conditions of callus and suspended cell culture conditions with the buds,young stems and young leaves from T.chinensis var as the explants.[Result]The effect was the best with the bud as the explants;the best way for sterilizing the explants of T.chinensis var mairei was：streptomycin detergent for 2 h＋suds for 3 h＋75% alcohol disinfection for 30 s＋10 % sodium hypochlorite solution for 25 min＋1 ‰ mercury chloride for 10 min;the optimum formula of callus subculture was：B5＋4.0 mg/L NAA＋0.5 mg/L 2.4-D＋0.2 mg/L GA＋0.5 mg/L 6-BA＋2 g/L AC.[Conclusion]This research built the high efficient regeneration system of T.chinensis var.