茶皂甙的提取工艺及天然茶果香波的研制

Extraction of Tea Saponins and Develop of Natural Tea Fruit Shampoo

[目的]研究从茶籽饼粕中提取茶皂苷的最佳工艺,并以茶皂苷为主要功能成分研制天然茶果香波。[方法]采用正交试验设计确定茶皂苷最佳提取工艺中的乙醇浓度、提取温度和提取时间;采用灌注法测定茶皂苷对浅部真菌的抑制作用;采用动物急性皮肤刺激性试验和人体斑贴试验测定天然茶果香波的刺激性和致敏性;按照国家标准GB11432-89检测天然茶果香波的产品质量。[结果]茶皂苷提取最佳工艺为:50%(V/V)乙醇于55℃下浸提5.3 h,所得浸膏中茶皂甙的含量为20.86%;此茶皂甙对人体浅部真菌有明显的杀灭和抑制作用;以茶皂苷浸膏为原料配制的茶果香波对正常人体皮肤无刺激性和致敏性;其理化指标均已达到GB11432-89标准。[结论]以茶皂苷为主要原料配制的天然茶果香波安全可靠,质量稳定,去屑止痒效果好。

[Objective] To study the optimum conditions of extract saponins from tea seed meal and to develop a natural tea fruit shampoo on this bases.[Method] Orthogonal test was used to determine the value of the ethanol concentration,extraction temperature and extraction time in the best extraction process.Perfusion method was used to determine its inhibition on the superficial fungi.Animal skin acute irritation test and human patch test were used to determine the shampoo＇s irritation and sensitization respeitively.National standard GB11432-89 was taken to test the shampoo＇s quality.[Result] The optimum conditions were extract with 50%（v/v） ethanol at 55 ℃ for 5.3 h and tea saponins yield was 20.86%.Meanwhile,tea saponins significantly inhibited human superficial fungal.The natural tea fruit shampoo had no irritation and sensitization on normal human skin and its physical and chemical indicators had reached the standard of GB11432-89.[Conclusion] The natural tea fruit shampoo was proved safe,reliable,stable quality and with good effect on antipruritic.