摘要
目的 评价七氟醚后处理对大鼠心肌缺血再灌注时Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶活性的影响,探讨其减轻心肌缺血再灌注损伤的机制.方法 健康雄性Wistar大鼠45只,体重250～280 g,随机分为3组（n=15）：假手术组（S组）、缺血再灌注组（I/R组）和七氟醚后处理组（Spo组）.I/R组和Spo组采用结扎左冠状动脉前降支30 min时进行再灌注的方法制备心肌缺血再灌注模型,S组仅在左冠状动脉前降支下穿线.Spo组再灌注前1 min开始吸入2.5%七氟醚持续5 min.于再灌注2 h时取心肌组织,测定心肌梗死体积、Na＋-K＋-ATP酶活性和Ca2＋-Mg2＋-ATP酶活性.结果 与S组比较,I/R组心肌梗死体积扩大,心肌组织Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的活性降低（P＜0.05）;与I/R组比较,Spo组心肌梗死体积缩小,心肌组织Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的活性升高（P＜0.05）.结论七氟醚后处理可提高Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的活性,从而减轻大鼠心肌缺血再灌注损伤.
Objective To investigate the effect of sevoflurane postconditioning on the activities of Na＋ -K＋ -ATPase and Ca2＋ -Mg2＋ -ATPase during myocardial ischemia-reperfusion （I/R） in rats and the possible mechanism. Methods Forty-five healthy male Wistar rats weighing 250-280 g were randomly divided into 3 groups （ n = 15 each）： sham operation group （group S）, I/R group and sevoflurane postconditioning group （group Spo）. Myocardial I/R was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 120 min of reperfusion. In group S the anterior descending branch was only exposed but not ligated. Group Spo received 5 min inhlation of 2.5% sevoflurane 1 min before reperfusion. The myocardial tissues were taken at 2 h of reperfusion for determination of infarct size and activities of Na＋ -K＋ -ATPase and Ca2 ＊ -Mg2 ＊ -ATPase. Results The infarct size was significantly larger and the activities of Na＋ -K＋ -ATPase and Ca2＋ -Mg2＋ -ATPase were signifi cantly lower in group I/R than in group S （ P 〈 0.05）. The infarct size was significantly smaller and the activities of Na＋ -K＋ -ATPase and Ca2＋ -Mg2＋ -ATPase were significantly higher in group Spo than in group I/R （P 〈 0.05 ）. Conclusion Sevoflurane postconditioning can reduce myocardial I/R injury through increasing the activities of Na＋ -K＋ -ATPase and Ca2＋ -Mg2＋ -ATPase.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第10期1179-1181,共3页
Chinese Journal of Anesthesiology
