同神经毁损药对大鼠坐骨神经运动神经功能的影响

Effects of different neurolytic drugs on function of motor nerve fibers of rat sciatic nerve

目的 评价不同神经毁损药物对大鼠坐骨神经运动神经功能的影响.方法 SD大鼠35只,随机分为7组（n=5）：正常对照组（C组）、阿霉素组（Ad组）、无水乙醇组（Aa组）、8%酚甘油组（Pg1组）、10%酚甘油组（Pg2组）、12%酚甘油组（Pg3组）和庆大霉素组（Ci组）.于臀肌间隙显露左侧坐骨神经,在坐骨神经穿入半膜肌和内收肌处注射相应药液各0.2 ml,C组：0.9%生理盐水,Ad组：5 mg/ml阿霉素,Aa组：无水乙醇,Pg1组：8%酚甘油,Pg2组：10%酚甘油,Pg3组：12%酚甘油,Ci组：4000 U/ml庆大霉素.于注药后21 d记录复合肌肉诱发电位波幅,并计算运动神经传导速度.结果 各组感觉神经传导速度均为0.与C组比较,其余各组运动神经传导速度和复合肌肉诱发电位波幅均明显降低（P＜0.05）;Ad组运动神经传导速度和复合肌肉诱发电位波幅明显高于其它药物组（P＜0.05）;Aa组及Pg3组运动神经传导速度和复合肌肉诱发电位波幅明显低于其余各组（P＜0.05）.结论与无水乙醇、酚甘油及庆大霉素相比,5%阿霉素对大鼠运动神经功能的影响较小,提示其更适合作为神经毁损药物.

Objective To investigate the change in the function of the motor nerve fibers of the sciatic nerve of rat following injection of different neurolytic drugs. Methods Thirty-five SD rats weighing 350-380 g were randomly divided into 7 groups （ n = 5 each）： group C received normal saline; group Ad adriamycin 5 mg/ml;group Aa anhydrous alcohol; group Pg1 8% phenol-glycerol; group Pg2 10% phenol-glycerol; group Pg3 12% phenol-glycerol and group Ci cidomycin 4000 U/ml. 0.2 ml of the different neurolytic drugs was injected at the points where the branches of the sciatic nerve entering semi-membranous and adductor magnus muscles. The action potential and conduction velocity of motor and sensory nerve fibers were measured at 21 d after injection. Results The conduction velocity of sensory nerve fibers was 0 in each group. The conduction velocity and action potential of the motor nerve fibers were significantly decreased in group Ad, Aa, Pg1, Pg2, Pg3 and Ci as compared with group C （the control group）. The action potential and the conduction velocity were significantly higher in group Ad than in other neurolytic drug groups. The potential and the conduction velocity were the lowest in group Aa and Pg3.The conduction velocity in group Pg1, Pg2, Ci was comparable. Conclusion 5% adriamycin seems to be a better neurolytic drug with less interference with function of motor never fibers.