摘要
目的 观察并探讨人滋养层细胞表面抗原2（Trop-2）在人胰腺癌中的表达及其临床意义.方法 用RT-PCR检测8例胰腺癌组织中Trop-2 mRNA的表达,免疫荧光检测人胰腺癌细胞系BxPc3中Trop-2蛋白的表达;免疫组化方法检测Trop-2蛋白在31例人胰腺癌组织及其癌旁组织芯片上的表达情况.统计分析Trop-2的表达与相应临床病理特征之间的关系.结果 胰腺癌组织中Trop-2 mRNA有表达,Trop-2蛋白主要表达在胰腺癌细胞膜上;Trop-2在人胰腺癌旁组织中的阳性表达率为9.7%,胰腺癌中阳性率为87.1%,其阳性表达程度随癌组织分化程度减低而增高（P＜0.01）;Trop-2的表达与患者年龄、性别无关.结论 Trop-2的表达与人胰腺癌的发展及恶性程度有关,可作为临床检测胰腺癌恶性程度标记,也可作为靶向治疗胰腺癌的潜在靶点。
Objective To investigate the expression and its clinical significance of Trop-2 in human pancreatic cancer. Methods Reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescence assay were used to characterize the expression of Trop-2 in human pancreatic cancer.The expression of Trop-2 protein in 31 tumor tissue samples, including peritumoral tissue from patients with pancreatic cancer, was detected with immunohistochemisty by tissue microarray. The clinicopathological characteristics and the tissue expression of Trop-2 protein were analyzed statistically. Results RT-PCR,immunofluorescence assay and immunohistochemisty by tissue microarray showed a high expression of Trop-2 in pancreatic cancer. The expression rate of Trop-2 was much higher in pancreatic cancer than that in peritumoral tissues （87. 1% vs 9. 7% ）. And a high expression of Trop-2 in pancreatic cancer was correlated with the low-differentiated changes. It had statistical significance （ P 〈 0. 05 ）. In contrast, no statistically significant correlation was found between the expression of Trop-2 and gender or age. Conclusion The expression of Trop-2 is correlated with the development and malignancy of pancreatic cancer. As a clinical prognostic marker, Trop-2 may be a potential therapeutic target for pancreatic cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第2期103-106,共4页
National Medical Journal of China
