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Toll样受体mRNA在人肾癌细胞786—0和正常肾细胞HK-2中的表达及意义 被引量：2

Expression and clinical significance of Toll-like receptors in human renal carcinoma cell 786-0 and normal renal cell HK-2
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摘要 目的探讨Toll样受体（TLR）在肾癌发生、发展中的作用和意义。方法培养人肾癌细胞786-0（786-0实验组）和正常肾细胞HK-2（HK-2对照组）。实时荧光定量聚合酶链反应（FQ—PcR）检测TLR1～TLR10mRNA在786-0实验组及HK-2对照组细胞中的表达规律。结果786-0实验组和HK-2对照组细胞均表达TLRl～TLR6、TLR8～TLR10，而不表达TLR7。TLR1、TLR2、TLR4～TLR6、TLR8、TLR10在786--0实验组表达均高于HK-2对照组细胞[（4．40±0．19）×10^-2、（1．77±0．24）×10^-2、（5．03±0．62）×10^-2、（3．93_4-0．86）×10^-2、（6．11±0．48）×10^-2、（1．89±0．47）×10^-2、（2．84±0．57）×10^-2比（0．84±0．27）×10^-2、（0．40±0．04）×10^-2、（0．95±0．73）×10^-2、（0．56±0．25）×10^-2、（0．49±0．16）×10^-2、（0．20±0．07）×10^-2、（0．27±0．08）×10^-2，P〈0．01]，而TLR3、TLR9在786-0实验组与HK-2对照组细胞的表达差异无统计学意义（P〉0．05）。结论TLR在人肾癌细胞和正常肾细胞细胞中的表达有差异，TLR1、TLR2、TLR4～TLR6、TLR8、TLR10在人肾癌的发生、发展中可能发挥一定的作用。 Objective To investigate the expression of toll-like receptors （TLR1-TLR10） in human renal carcinoma cell 786-0 and normal renal celI HK-2 and discuss the significance of TLRs in the development of renal carcinoma. Methods Human renal carcinoma cell 786-0 （ experimental group） and normal renal cell HK-2 （control group） were cultured. The expression of TLR1-TLR10 was detected by real- time fluorogenic quantitative PCR （FQ-PCR）. Results The FQ-PCR analysis demonstrated that 786-0 cell line and HK-2 cell line expressed TLR1-TLR6 and TLR8-TLR10 mRNA. Yet neither had an expression of TLR7. Moreover, the expression levels of TLR1, TLR2, TLR4, TLRS, TLR6, TLR8 and TLR10 in the experimental group were significantly higher than those in HK-2 cell line [（4.40±0.19）×10^-2、（1.77±0.24）×10^-2、（5.03±0.62）×10^-2、（3.93_4-0.86）×10^-2、（6.11±0.48）×10^-2、（1.89±0.47）×10^-2、（2.84±0.57）×10^-2 vs（0.84±0.27）×10^-2、（0.40±0.04）×10^-2、（0.95±0.73）×10^-2、（0.56±0.25）×10^-2、（0.49±0.16）×10^-2、（0.20±0.07）×10^-2、（0.27±0.08）×10^-2,P〈0.01]. There was no significant change in the expressions of TLR3 and TLR9 between 786-0 and HK-2 cell line （P 〉 0. 05）. Conclusion The expressions of TLR1, TLR2, TLR4, TLRS, TLR6, TLR8 and TLR10 are significantly higher in 786-0 cell line than those in HK-2 cell line. They may play key roles in the development of renal carcinoma.
作者 于慧 许闪闪 程钦全 何丽梅 李智
机构地区 同济大学附属第十人民医院检验科 苏州大学医学院部
出处 《中华医学杂志》 CAS CSCD 北大核心 2011年第2期129-131,共3页 National Medical Journal of China
基金 基金项目：国家自然科学基金青年基金（20902068） 上海市卫生局青年科研项目（2008Y020）
关键词 肾肿瘤 TOLL样受体 荧光定量PCR Kidney neoplasms Toll-like receptors Fluorogenic quantitative PCR
分类号 R737.11 [医药卫生—肿瘤]
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参考文献7

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二级参考文献3

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共引文献6

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中华医学杂志
2011年 第2期

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