谷氨酰胺对兔梗阻性黄疸肠粘膜屏障的保护作用

Protective effect of glutamine on impaired intestine mucosal barrier in rabbits with obstructive jaundice

目的观察谷氨酰胺对兔梗阻性黄疸肠粘膜屏障功能损害的保护作用。方法部分结扎胆总管制备梗阻性黄疸兔模型后,行静脉置管后注射丙氨酰谷氨酰胺1 mg.Kg-1(A组),假手术组(B组)和黄疸模型组(C组)给予生理氯化钠溶液。于术后24 h处死动物后检测静脉血内毒素水平和血清二胺氧化酶(d iam ineoxidase,DAO)变化,观察小肠粘膜上皮变化并进行组织病理评分。结果阻塞性黄疸组血清总胆红素较假手术组明显增高(P<0.05)。与C组比较,A组静脉血内毒素明显降低(P<0.05),血清二胺氧化酶明显降低(P<0.05),A组肠上皮粘膜病理学评分明显优于C组(P<0.05)。结论谷氨酰胺可保护梗阻性黄疸时小肠粘膜屏障功能,减少内毒素血症的发生,对改善预后有一定作用。

Objective：To investigate the protective effect of glutamine（Gln）on impaired intestine mucosalbarrier in rabbits with obstructive jaundice.Methods：After the obstructive jaundice was induced by ligation of cornmon bile duct,the rats were treated with alanyglutamine 1mg·Kg-1（Group A）,Group C was given the same amout of normal saline.At 24h after the end of treatments,the endotoxin and diamineoxidase of plasma were measured,the mucosa was observed,and the biography rate was made.Results：The indices related to the DAO,endotoxin level and biography rate were higher in Group C than those in Group A（P0.05）.Conclusion： The intestinal mucosal baeeier may be injuryed during the obstructive jaundice.The Gln has significant effect in protecting the mucosal barrier.