“通利大肠”对慢性阻塞性肺疾病大鼠气道黏液高分泌的影响

Influence of therapy of dredging large intestine on airway mucus hypersecretion in COPD rats

目的观察"通利大肠"对慢性阻塞性肺疾病(COPD)模型大鼠气道黏液高分泌的影响,从黏液高分泌角度探讨COPD"从肠论治"的效应机制。方法采用气管注脂多糖加熏香烟联合造模方法建立COPD大鼠模型,随机分为正常组、模型组、治肺组、治肠组及肺肠同治组。正常组、模型组灌胃生理盐水,各给药组灌胃相应中药,连续14 d。实时荧光定量PCR法及免疫组化法检测支气管组织水通道蛋白5(AQP5)mRNA、黏蛋白5AC(MUC5AC)mRNA水平及蛋白表达。结果与正常组比较,模型组大鼠气道AQP5 mRNA水平及蛋白表达减少,MUC5AC mRNA水平及蛋白表达增加(P<0.01)。与模型组比较,治肠组AQP5 mRNA水平及蛋白表达增强,MUC5AC mRNA水平及蛋白表达减少(P<0.01)。与治肺组比较,肺肠同治组AQP5 mRNA水平及蛋白表达增强,MUC5AC mRNA水平及蛋白表达减少(P<0.01)。结论通利大肠,或在治肺的基础上增加通利大肠,均能降低MUC5AC mRNA水平及蛋白表达,增强AQP5mRNA水平及蛋白表达,从而抑制气道黏液高分泌,这可能是COPD"从肠论治"效应产生的作用环节之一。

Objective To observe the influence of the therapy of dredging large intestine on airway mucus hypersecretion in rat model of chronic obstructive pulmonary disease（COPD）,and discuss the effective mechanism of treating COPD based on treating large intestine from the aspect of mucus hypersecretion.Methods The rat model of COPD was established using intratracheal injection of LPS combined with cigarette smoking.All rats were randomly divided into the normal group,model group,lung group,intestine group and lung-intestine group.The normal group and model group were given normal saline solution intragastrically and other groups were treated with corresponding medicinal for 14 days.The levels and expressions of aquaporin 5-mRNA（AQP5-mRNA） and MUC5AC-mRNA were detected by applying RT-PCR and immunohistochemistry assay.Results Compared with the normal group,in the model group the level and expression of AQP5-mRNA decreased,and level and expression of MUC5AC-mRNA increased（P0.01）.Compared with the model group,in the intestine group the level and expression of AQP5-mRNA increased,and level and expression of MUC5AC-mRNA decreased（P0.01）.Compared with the lung group,in the lung-intestine group the level and expression of AQP5-mRNA increased,and level and expression of MUC5AC-mRNA decreased（P0.01）.Conclusion The level and expression of MUC5AC-mRNA can be decreased and those of AQP5-mRNA can be increased,and airway mucus hypersecretion can be inhibited by the therapy of dredging large intestine,or dredging large intestine on the base of treating lung disease.It may be one effective joints of treating COPD based on treating large intestine.