茯苓化学成分分离与结构鉴定

Determination and isolation of the chemical constituents of Poria Cocos

目的研究茯苓中化学成分,阐明茯苓药效物质基础。方法采用稀乙醇提取,各种柱色谱法如硅胶、反相、凝胶等,以及结晶法进行分离纯化,用紫外光谱、核磁共振、质谱等各种光谱波谱技术对分离得到的化合物进行结构鉴定。结果从茯苓中分离得到10个化合物,分别鉴定为乙酰依布里酸(1),麦角甾醇过氧化物(2),麦角甾-7,22-二烯-3β,5α,6β-三醇(3),麦角甾-7-烯-3β醇(4),麦角甾-7,22-二烯-3β醇(5),茯苓酸(6),去氢土莫酸(7),3-表-去氢土莫酸(8),依布里酸(9),3β-羟基-羊毛甾-7,9(11),24-三烯-21-酸(10)。结论化合物1、2、3、4为首次从茯苓中分离得到。

Objective To study the chemical constituents of Fuling（Poria）.Methods The chemical constituents of Fuling were extracted with dilute alcohol,and isolated and purified with various chromatographic columns including silica gel,reversed phase and gelatin chromatography,and crystallization methods.The structure was indentified by using different spectroscopic and chromatographic techniques including ultraviolet spectrum,nuclear magnetic resonance and mass spectrum.Results There were 10 chemical constituents isolated from Fuling,including acetyl eburicoic acid（1）,ergosterol peroide（2）,cerevisterol（3）,ergosta-7-en-3β-ol（4）,ergosta-7,22-dien-3β-ol（5）,pachymic acid（6）,dehydrotumulosic acid（7）,3-epi-dehydrotumulosic acid（8）,eburicoic acid（9） and dehydrotrametenolic acid（10）.Conclusion The constituents 1,2,3 and 4 were isolated from Fuling for the first time.