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组蛋白赖氨酸甲基转移酶SET7/9对myogenin基因表达的影响 被引量：1

SET7/9 Enhances Myogenin Gene Transcription in C2C12 Cells
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摘要 目的利用本组构建的小鼠组蛋白赖氨酸甲基转移酶SET7/9真核表达质粒及RNA干扰(siRNA)质粒,在体外成肌细胞分化模型C2C12细胞中检测SET7/9对肌肉分化关键基因myogenin基因表达的影响。方法转染SET7/9真核表达质粒pCMV-3tag-6-SET7/9至C2C12细胞,Western blot技术验证其表达;利用实时荧光定量PCR(Real-time PCR)技术检测SET7/9对myogenin基因mRNA水平的影响;利用双荧光素酶报告系统检测SET7/9对myogenin基因启动子活性的影响;转染SET7/9的siRNA质粒,利用Real-time PCR技术检测其抑制效果以及对myogenin mRNA水平的影响。结果 SET7/9真核表达质粒pCMV-3tag-6-SET7/9可在C2C12细胞中有效表达;SET7/9可使myogenin mRNA水平升高并能够促进myogenin启动子活性升高;干扰SET7/9表达可使myogenin mRNA水平降低。结论组蛋白赖氨酸甲基转移酶SET7/9可激活肌肉分化关键基因myogenin的转录。 Objective Eukaryotic expression plasmid and siRNA plasmid of mouse histone lysine methyltransferase SET7/9 was used to investigate the effect of SET719 on expression of myogenin.Methods The eukaryotic expression of SET7/9 plasmid named pCMV-3tag-6-SET7/9 was transfected into C2C12 cells and identified by Western blot.The effect of SET7/9 on muscle differentiation marker gene myogenin mRNA expression was detected by Real-time PCR.The effect of SET7/9 on myogenin promoter activity was detected by Dual-luciferase reporter system.Real-time PCR was applied to detect the effect of SET7/9 siRNA plasmid on myogenin mRNA expression.Results SET7/9 promoted myogenin mRNA expression and increased myogenin promoter activity.In addition,myogenin mRNA level was inhibited by knockdown of SET7/9.Conclusion Histone lysine methyltransferase SET7/9 can promote transcription of muscle differentiation marker gene myogenin.
作者 潘炜松 代辉 张业 沈珝琲
机构地区 中国医学科学院基础医学研究所/北京协和医学院基础学院医学分子生物学国家重点实验室
出处 《医学研究杂志》 2011年第1期32-35,共4页 Journal of Medical Research
基金 国家自然科学基金资助项目(30721063 90919048)
关键词 SET7/9 肌肉分化 RNA干扰 MYOGENIN SET7/9 Muscle differentiation siRNA Myogenin
分类号 R738.7 [医药卫生—肿瘤]
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参考文献14

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二级参考文献10

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同被引文献7

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医学研究杂志
2011年 第1期

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