Netrin-1在HeLa细胞的表达及对HeLa细胞功能的影响

Expression of Netrin-1 and its effects on cervical cancer HeLa cell line

目的:研究Netrin-1在HeLa细胞的表达及对HeLa细胞增殖、侵袭和凋亡的影响。方法:利用蛋白质印迹法检测Netrin-1在HeLa细胞的表达,并通过MTT、侵袭实验和流式细胞仪分别检测10,50和100ng/mlNetrin-1对HeLa细胞增殖能力、侵袭能力和凋亡的影响。结果:(1)Netrin-1在HeLa细胞有表达,其相对表达量为0.389±0.026;(2)MTT实验结果显示,Netrin-1可以促进HeLa细胞增值,表现为时间和剂量依赖性,与对照组有显著差异(P<0.05或P≤0.01);(3)Netrin-1处理组细胞迁移数分别为314±16,487±15和553±19,均明显多于对照组180±10(P均<0.05);(4)在Netrin-1干预下,HeLa细胞凋亡率呈递减趋势,与对照组比较有显著差异(P<0.05或P<0.01)。结论:HeLa细胞表达的Netrin-1与宫颈癌的侵袭转移有关,Netrin-1促进HeLa细胞增殖和侵袭能力,并抑制HeLa细胞凋亡,可能为未来宫颈癌的治疗提供依据。

Objective：To study the expression of Netrin-1 in HeLa cells and assess its effects on the viability,invasion and apoptosis of HeLa cells.Methods：Western blotting was used to determine the expression of Netrin-1 in HeLa cells.MTT assay and invasion assay were conducted.The apoptosis were detected by flow cytometry（FCM） assay.Results：（1）The expression of Netrin-1 was detected in HeLa cells（0.389±0.026）.（2） Compared to the control,10,50 and 100ng/ml Netrin-1 treatments of HeLa cells caused a significant increase in cells proliferatin in a concentration-and time-dependent manner（P0.05,P0.01,respectively）.（3） Cell invasion assay revealed that the number of migrating HeLa cells induced by Netrin-1 was also increased significantly（314±16,487±15,553±19 vs 180±10,P0.05）.（4） After treatment,total cells apoptosis significantly decreased（P0.05 and P0.01）.Conclusion：Netrin-1 can enhance proliferation,invasion and inhibitition of apoptosis in HeLa cells.This information from this study may serve as a basis future developmental therapeutics of cervical cancer.