核转录因子和趋化因子受体-4在肾癌中的表达及意义

Expression and significance of NFκB and CXCR4 in renal cell carcinoma

目的检测核转录因子（NFκB）和趋化因子受体-4（CXCR4）在肾癌组织中表达情况,探讨两者在肾癌发生和进展过程中的意义。方法运用免疫组织化学方法检测43例肾癌组织和21例癌旁组织标本中NFκB（p65）和CXCR4阳性表达情况,分析两者与临床、病理参数的联系以及两者之间的相关性。结果 NFκB（p65）在33例（76.7%）肾癌组织和10例（47.6%）癌旁组织中表达阳性;CXCR4在29例（67.4%）癌组织和7例（33.3%）癌旁组织中表达阳性。两者于两组织中阳性表达上分别存在显著差异。NFκB（p65）与CXCR4均表达于所有Ⅲ~Ⅳ临床分期肾癌组织（100%）,显著高于Ⅰ~Ⅱ期阳性表达率（66.7%与53.3%）,而阳性表达在肾癌的不同病理分级、年龄、性别上无差异。同时,NFκB（p65）与CXCR4在肾癌中的表达之间存在相关性（r=0.44）。结论 NFκB与CXCR4与肾癌的发生和进展密切相关,两者之间可能存在调节关系。因此,NFκB与CXCR4可成为预防和治疗肾癌及其转移的靶点。

Objective To detect the expression of NFκB and CXCR4 in renal cell carcinoma（RCC） and discuss the significance of the two cytokines for the development and progression of RCC.Methods Immunohistochemistry（IMC） was employed to inspect the expression of p65/NFκB and CXCR4 protein in 43 cases of renal cell carcinoma（RCC） and 21 adjacent renal tissues.The correlation between the expression of the two cytokines,and the clinic/pathologic parameter,were analysed.Results NFκB（p65） was positvely expressed in 33（76.7%） RCCs and 10（47.6%） adjacent renal tissues;CXCR4 was positvely expressed in 29（67.4%） RCCs and 7（33.3%） adjacent renal tissues.The positive expression of NFκB（p65） and CXCR4 was obviously diferent between RCC and adjacent renal tissues.NFκB（p65） and CXCR4 were expressed in all RCCs in Ⅲ-Ⅳ clinic-stage（100%）,which was obviously higher than in the Ⅰ-Ⅱ clinic-stage（66.7% and 53.3%）,but they did not correlate with pathological grades,the age and gender of patients.Furthermore,a correlation existed between NFκB（p65） and CXCR4 in the RCCs（r=0.44）.Conclusion NFκB and CXCR4 closely correlated with the development and progression of RCC and there might existe a regulating correlation between them.Therefore,they could become a potential therapeutic and preventive targetets and metastasis of RCC.