云南汉族人群5-羟色胺转运体基因启动子区多态性与酒精依赖的相关性

Association of polymorphism in the serotonin transporter gene promote with the susceptibility to alcohol dependence in Yunnan Han Population

为了探讨云南汉族人群中5-羟色胺转运体基因启动子区多态性(5-HTTLPR)与酒精依赖的关联性,文章采用PCR扩增和DNA测序技术,对云南地区118例酒精依赖患者和214例健康对照个体进行了5-HTTLPR的基因多态性分析。结果表明:酒精依赖患者组和正常对照组的5-HTTLPR的基因型分布存在显著性差异,L/L和L/S基因的携带者人群嗜酒发生率显著低于S/S基因型人群(OR:0.581,P=0.026)。S和L等位基因频率在两组间无统计学差异(χ2=2.594,P=0.107),但其分布存在种族差异性。因此,云南地区人群中5-HTTLPR多态与酒精依赖存在相关性,L/L和L/S基因型可能是降低酒精依赖发病的影响因子之一。

To explore the association of polymorphism in the serotonin transporter gene and the susceptibility to alcohol dependence in Yunnan Han population,PCR and DNA sequencing techniques were used to detect 5-HTT-linked promoter region（5-HTTLPR）.One hundre and eighteen alcohol dependent patients as case group and 214 normal people as control group were employed in this study.Significant differences in genotype frequencies were present between case group and control group of 5-HTTLPR（P0.05）.The proportion of L/L and L/S genotype was significantly smaller in case group than that was in control group（OR=0.581,P=0.026）.No significant association was observed in allelic frequencies,which differed in different ethnic groups.In conclusion,5-HTTLPR polymorphism may be associated with alcohol dependent patients,and the genotype L/L or L/S may be a genetic factor that is responsible for decreasing susceptibility of alcohol dependence in Yunnan Han population.