错配修复基因hMSH2IVS12-6T>C多态性与胃癌危险性的研究

The relationship between hMSH2IVS12-6T>C polymorphism and the risk of gastric cancer

目的 探讨碱基错配修复基因hMSH2IVS12-6T〉C多态性与江苏宜兴人群胃癌易感性之间的关系.方法 采用分子流行病学病例对照研究方法,以552例胃癌患者和592例年龄（±5岁）、性别相匹配的非胃癌患者（对照组）作为研究对象,用 TaqMan MGB （minor grove binder） 探针对hMSH2IVS12-6T〉C多态性进行基因分型,分析不同基因型与胃癌发生风险的关联性,通过分层分析探讨年龄、性别、吸烟及饮酒等因素对罹患胃癌的影响.结果 与hMSH2IVS12-6TT基因型相比,TC和CC基因型均与胃癌发生风险无显著关联性（P=0.882和P=0.569）.合并突变基因型TC＋CC并不显著降低胃癌的发生风险（调整OR=0.96, 95%CI=0.75～1.23, P=0.756）.分层分析结果显示吸烟、饮酒、性别、年龄等因素与胃癌的发生风险亦无显著关联性.结论 hMSH2IVS12-6T〉C多态性与江苏宜兴人群胃癌发生风险无显著关联.

Objective To explore the association of mismatch repair gene hMSH2IVS12-6T 〉 C polymorphism and the risk factors of gastric cancer patients in Yixing city. Methods In a hospital-based case-control study of 552 patients with gastric cancer and 582 cancer-free controls frequency-matched by age （ -＋ 5 ） and sex, we genotyped the IVS12-6T 〉 C polymorphism of hMSH2 gene by Taqman MGB probe method. We further assessed its association with risk of gastric cancer and interaction with age, sex, smoking status and alcohol use. Results Compared with the wild-type homozygote, no significant association was observed between the hM- SH2IVS12-6T 〉 C polymorphism and gastric cancer risk（ P = 0. 882 and P = 0. 569 respectively） and the combined variant genotypes （TC ＋ CC） had not significantly decrease the risk of gastric cancer （OR =0.96, 95% CI = 0.75 - 1.23, P = 0. 756）. We also found no significant association between the variant genotypes of the hMSH2IVS12-6T 〉 C polymorphism and the age, sex, smoking status and alcohol use. Conclusions There was no significant association between the hMSH2IVS12-6T 〉 C polymorphism and gastric cancer susceptibility in Yixing population which we selected.