姜黄素固体脂质纳米粒的制备及表征

Preparation and characterization of curcumin solid lipid nanoparticles

目的:制备姜黄素(Cur)固体脂质纳米粒(SLN)。方法:用薄膜超声法制备Cur-SLN,以mcur:m单硬脂酸甘油酯、m单硬脂酸甘油酯:m卵磷脂、聚山梨酯-80质量浓度、超声时间为考察因素,以包封率为指标,用正交试验优选处方,并考察其粒径分布、Zeta电位。结果:当mcur:m单硬脂酸甘油酯=1:3、m单硬脂酸甘油酯:m卵磷脂=1:2.5、聚山梨酯-80质量浓度2.5%、超声时间12min时,所制得的Cur-SLN平均粒径为(145.6±5)nm,Zeta电位为(-31.9±1.5)mV,包封率为(97.42±0.39)%,载药量为(7.92±0.05)%。结论:采用薄膜-超声法制备Cur-SLN可行,为开发姜黄素新型给药系统提供试验依据。

Objective：To optimize technique for preparing curcumin solid lipid nanoparticles（Cur-SLN） by film-ultrasonic dispersion.Methods：The influences of various factors,such as the ratios of curcumin and monostearin,monostearin and lecithin.Taking the concentration of Tween-80 and ultrasonic time（min） as on factors and the entrapment efficiency as index,the optimum formula was selected and particle size distribution and Zeta potential were studied by orthogonal test.Results：The optimized preparation conditions were as follows：mcur-mmonostearin = 1：3;mmonostearin-mlecithin = 1：2.5;The concentration of Tween-80 was 2.5%;Ultrasonic time was 12 min.The average particle size diameter was（145.6 ± 5） nm;Zeta potential was（-31.9 ± 1.5） mV;Entrapment efficiency was（97.42 ± 0.39） %;Drug loading was（7.92 ± 0.05）%.Conclusion：It is feasible to prepare Cur-SLN by film-ultrasonic dispersion techniques and the study results provide the test evidence for developing new curcumine drug delivery system.