IL-1和IL-6在脊髓损伤大鼠神经细胞中的表达及其动态变化

Dynamic Change of Expression and Distribution of IL-1 and IL-6 in Rat Neuron Following Spinal Cord Injury

目的探讨SD大鼠脊髓损伤部位神经细胞中IL-1和IL-6表达的动态变化及其意义。方法采用ELISA法检测大鼠脊髓组织匀浆内IL-1、IL-6含量;用免疫荧光双标记技术,在荧光显微镜下观察脊髓组织切片中IL-1、IL-6在脊髓神经细胞内的表达及其与神经丝蛋白(NF)共定位情况。结果①脊髓损伤后,IL-1、IL-6除在胶质细胞表达外,在脊髓神经细胞也有较强表达,且明显高于正常对照组;此外,假手术组也明显高于正常对照组。②ELISA检测结果表明:脊髓损伤组细胞因子IL-1和IL-6在脊髓损伤后1h内就迅速升高,至第6h达到第一个高峰,而后下降,而假手术组在第12h达第一个高峰;至第1d后损伤组和假手术组都逐渐升高,至第5d达到第二个高峰,第7d后稍下降,但仍高于正常对照组。结论脊髓损伤后,脊髓组织内神经胶质细胞和神经细胞均能表达大量的细胞因子IL-1、IL-6,参与机体炎症反应,推测脊髓灰质内的神经细胞可能具有调节脊髓损伤局部炎症反应的生物学功能。

Objective To explore the dynamic change of expression of IL-1 and IL-6 in rat neuron following spinal cord injury（SCI）.Methods With Double-label immunofluorescence method,we observed,by confocal microscopy,the distrbutions of pro-inflammatory such as interleukin 1 and interleukin 6 in the neurons labeled by NF.With ELISA technology,we quantitively detected expressions of IL-1 and IL-6 in rat spinal cord samples at various periods,and analyzed the curves of dynamics in three experimental groups during the above periods.Results ① pro-inflammatory（IL-1 and IL-6） significantly were expressed in neurons following spinal cord injury.②The expression level increased with time-lapse,and the first peak of IL-1 presented at 6 h while IL-6 presented at 12h after injury,which was followed by decreasing to the normal basic level at 24h.Subsequently,the secend peak showed at the 3 th day after injury,and the expression level began to decrease at the seventh day.Conclusion We suggested that neurons,in addition to glias,might initiate the innate immunity and play certain role in CNS immunoreactions after SCI.Moreover,we assumed that the stimulations might directly activate the neurons and glias,and resulted in the first peaks of expression of several cytokines,while the second peak might result from the secondary reactions.The results would shed light on the approach to the resarch of clinical therapy on SCI.