COX-2、p53和c-myc在皮肤肿瘤中的表达及意义

The expression and significance of COX-2、p53 and c-myc in cutaneous tumors

目的利用组织芯片检测Cox-2、p53和c-myc在皮肤鳞状细胞癌、基底细胞癌和Bowen病中的表达,探讨它们在上述皮肤肿瘤发病中的意义。方法手工制作组织芯片,用免疫组织化学技术检测30例皮肤SCC,21例BCC,20例BD的石蜡包埋组织及10例正常皮肤组织中COX-2、p53和c-myc的表达。结果 COX-2蛋白在皮肤肿瘤组织中阳性率明显高于正常皮肤对照组,两者差异有统计学意义(P<0.01);p53蛋白在皮肤肿瘤组织中阳性率高于正常皮肤对照组,两者差异有统计学意义(P<0.05);c-myc蛋白在皮肤肿瘤组织中阳性率高于正常皮肤对照组,两者差异有统计学意义(P<0.05);COX-2、p53和c-myc相互之间在所检测皮肤肿瘤中的表达无相关性(P均>0.05)。结论组织芯片适合于样本量较大的研究。COX-2在皮肤SCC、BCC、BD中呈高表达,其与皮肤SCC、BCC、BD的发生关系密切。p53在皮肤SCC、BCC、BD中呈高表达,其与皮肤SCC、BCC、BD的发生关系密切。c-myc在皮肤SCC、BCC中呈高表达,与皮肤BD的发生无明显关系。三者均可作为判定上皮源性皮肤肿瘤恶性程度的一种标志。COX-2、p53和c-myc相互之间在所检测皮肤肿瘤中的表达水平无相关性,具体机制尚不清楚。

Objective To explore the expression of COX-2、p53 and c-myc in squamous cell carcinoma,basal cell carcinoma,Bowen′s disease.To study the clinical significance in cutaneous tumors by tissue microarray.Methods 30 cases of SCC,21 cases of BCC,20 cases of Bowen′s disease and 10 cases of normal skin tissue were detected by immunohistchemical technique with tissue microarray for the expression of COX-2、p53 and c-myc.Results The positive rate of COX-2 in cutaneous tumor was obviously higher than normal skin tissue（P0.01）.The positive rate of p53 in cutaneous tumor was higher than normal skin tissue（P0.05）.The positive rate of c-myc protein in cutaneous tumor was higher than normal skin tissue （P0.05）.The level of expression of COX-2、p53 and c-myc in cutaneous tumor have no associativity each other.Conclusion Tissue microarray is satisfy the need of foundational and clinical researcher.COX-2 is high expression in skin SCC、BCC and Bowen′s disease,and COX-2 has close relationship with the occurrence of cutaneous tumor.p53 is high expression in skin SCC、BCC and Bowen′s disease,the mutation of p53 has close relationship with the occurrence of cutaneous tumor.C-myc is high expression in skin SCC and BCC,has no relationship with Bowen′s disease.All of these were considered as mark in assessment of malignant tumor level.Finally,the level of expression about COX-2、p53 and c-myc in cutaneous tumor have no associativity with each other,but the mechanism was unknown now.