有氧运动对大鼠慢性酒精性肝损伤的影响

Protective effect of aerobic exercise on rat liver injury caused by chronic alcohol intakes

目的:探讨有氧运动对单纯性酒精性肝损伤的保护作用。方法:雄性SD大鼠36只,随机分为正常对照组、模型对照组和模型运动组,每组12只。模型组以54%酒精灌胃建立大鼠慢性酒精性肝病模型,正常对照组灌胃等量的生理盐水,模型运动组大鼠同时进行无负重游泳训练。10周后全部处死,测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性,肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,同时光镜下观察肝组织形态学改变。结果:模型对照组大鼠血清AST、ALT水平较正常对照组显著升高(P<0.01或P<0.05),肝组织SOD活性明显降低(P<0.05)、MDA含量明显升高(P<0.01)。运动组与模型对照组相比,血清AST有明显下降(P<0.05),肝组织SOD活性升高(P<0.05)、MDA含量下降(P<0.05)。HE染色显示,模型对照组出现明显的脂肪变性,可见不同程度的点灶状或片状坏死,模型运动组肝脂肪变性和炎症程度轻于模型对照组。结论:有氧运动能增强肝组织清除氧自由基的能力,降低肝组织脂质过氧化反应,从而减轻酒精对肝脏的损伤,促进肝功能的恢复。

Objective： To study the protective effect of aerobic exercise on liver injury caused by chronic alcohol intakes.Methods： 36 male SD rats were randomly divided into three groups： normal control group,model control group and model exercise group.The rats in the model group were administered intragastrically with 54% alcohol,while the ones in the normal control group with physiological saline.The rats in the model exercise group were performed swimming training.After 10 weeks,the activity of ALT,AST in serum and the content of MDA and SOD in liver were measured for each sample respectively.The livers of all rats were observed histologically.Results： The activity of ALT,AST in serum of the model control group was significantly higher（P0.01 or P〈0.05）,the activity of SOD in liver was significantly lower（P〈0.05）but the concentration of MDA in liver was significantly higher（P〈0.01）than those of the normal control group.After 10 weeks of swimming exercise,the activity of AST in serum was decreased significantly（P〈0.05）,the activity of SOD in liver was significantly increased（P〈0.05）and the concentration of MDA in liver was significantly decreased（P〈0.05）as compared with the model control group.The pathological changes of liver tissue were observed with H.E staining.Steatosis and inflammation were prominent in alcohol-treated rat liver,and there was piecemeal or focal necrosis as well.Furthermore,less pathological changes were found in the model exercise group than those in the model control group.Conclusion：The aerobic exercise can enhance the metabolism of free radicals.The results indicate that aerobic exercise has some protective effect on chronic alcohol-induced injury on rat liver and can encourage the functional recovery of liver.