摘要
目的:建立香果健消片中木香主要成分的鉴别及含量测定方法。方法:采用薄层色谱法鉴别木香;高效液相色谱法测定木香烃内酯和去氢木香内酯的含量。结果:薄层色谱法鉴别斑点明显,木香烃内酯和去氢木香内酯进样量在0.10～2.00μg范围内与峰面积的线性关系良好。加样回收率分别为99.3%(RSD=1.24%,n=6)和100.8%(RSD=1.67%,n=6)。结论:上述方法专属性强、重复性好,可用于木香的鉴别、木香中木香烃内酯及去氢木香内酯的含量测定。
Objective： To establish a method for identification of Costustroot and determination of costunolide and dehydrocostus lactone in Xiangguojianxiao tablets.Methods： Costustroot was identified by TLC;the contents of costunolide and dehydrocostus lactone were determined by HPLC.Results： The developed TLC spots were quite clear;the calibration curve of costunolide and dehydrocostus lactone were linear within 0.10-2.00 μg.The average recoveries of costunolide and dehydrocostus lactone were 99.3% with RSD of 1.24%（n=6） and 100.8% with RSD of 1.67 %（n=6）,respectively.Conclusion： The established methods are specific,reproducible and suitable for the identification of costustroot and determination of costunolide and dehycostus lactone.
基金
大理学院大学生科研基金资助项目(2009DXSY01)
