PCR技术在甘蔗病害检测中的应用

Application of PCR based disease detection methods in sugarcane

甘蔗病害是制约甘蔗产业稳定可持续发展的主要因素之一,而病害检测在甘蔗育种、健康种苗繁育以及植物检疫等方面起着重要的作用。文章综述了PCR技术在甘蔗真菌性病害、细菌性病害、病毒性病害以及植原体病害检测中的应用概况,并列出相应的检测引物,为相关科研人员检测甘蔗病害提供参考。

Sugarcane is the most important sugar crop worldwide, the most important constraints in its production and development of new varieties are the biotic stresses （diseases）, which affect its productivity all over the world. For sustainable sugarcane and sugar production, the management of disease, in terms of detection and application of control measures, plays a pivotal role, and is the key for developing new variety and producing virus-free seedcane and plant quarantine applications. This paper reviews the application of PCR in detection of major fungal, bacterial, virus and phytoplasma diseases of sugarcane. Further, a list of primers for PCR detection of some most important diseases, occurring in China, have also been provided for the researchers involved in disease management for the sugarcane crop.