矮岩羊乳酸脱氢酶同工酶的分离和测定

SEPARATION AND QUANTITATION OF LACTIC DEHYDROGENATE ISOZYMES OF DWARF BLUE SHEEP (PSEUDOIS SCHAEFERI)

本文采用聚丙烯酰胺凝胶圆盘电泳法,对矮岩羊(Pseudois Schaeferi)的血清、肝、肾、肺、骨骼肌、心肌和小肠的乳酸脱氢酶(LDH)同工酶进行分离和测定.其结果显示:成体和幼体矮岩羊各种组织中LDH同工酶的分布与含量,既具有相类似的特点又有一些差异,且与人和某些脊椎动物的特点有很大的差异。

In order to study the characteristics of the glyeolysis of the rare animal Dwarf Blue Sheep (pseudois schaeferi), by way of polyaerylamide dies gel electrophoresis we separated, quantitated the lactic dehydrogenase (LDH)isozymes of the serum, liver, kidney, lungs and skeletal muscle, cardiac muscle and small intestine of Dwarf Blue Sheep. The results indicate that the distribution and content of the LDH isozymes in all tissues of adult and young Dwarf Blue Sheep share some charateristics in common and also differ in certain respects. And when compared with that of man and some vertebrate, the above mentioned characteristics are notably different.