泰州市奶牛乳房炎调查及其病原菌的分离

Investigation of Dairy Cows Mastitis and Isolation the Pathogenic Bacteria in Taizhou Region

奶牛乳房炎是奶牛场的常发病和难以防治的疾病,为进一步了解引起奶牛乳房炎的病原微生物,本研究通过对泰州市3个奶牛场572头泌乳牛的乳房炎发病率进行了调查并对乳房炎主要病原菌进行分离和鉴定。结果表明,奶牛临床型乳房炎的发病率为7.69%,隐性型乳房炎发病率为56.64%,乳区阳性率为33.8%,其中临床型:隐性=1:7.36。从65份确定患有乳房炎的奶样中共分离获得8种细菌、156株分离株,且引起乳房炎的主要病原菌是金黄色葡萄球菌和表皮葡萄球菌,其次是停乳链球菌、大肠杆菌、乳房链球菌和无乳链球菌,其检出率分别为42.31%、23.08%、8.97%、6.41%、5.13%和1.28%。该项调查结果初步明确了泰州市乳房炎发病情况,同时为进一步综合防治奶牛乳房炎和研制乳房炎治疗药物提供了科学依据。

Dairy cows mastitis is a commonly-occurring and difficult-prevention disease,for further information on pathogens causing dairy cows mastitis,in this study,we investigated the rate of dairy cow mastitis on 572 milking cows of 3 dairy farms in Taizhou,at the same time,we isolated and identified the main pathogenic bacterium on mastitis.The results showed that the incidence rate of the clinical mastitis was 7.69%,while the incidence rate of the recessive mastitis was 56.64%,the positive rate of quarters was 33.8%,among which the rate between the clinical mastitis and the recessive mastitis was 1：7.36.Further results indicated that 8 bacteria,156 isolates were isolated from 65 samples which have been identified suffering from mastitis.The main pathogenic bacterium on mastitis was Staphylococcus aureus,the Staphylococcus epidermidis and the Staphylococcus saprophyticus with the rate of isolating 42.31%,23.08% and 6.41%,respectively.However,the isolating rate of the Streptococcus dysgala-ctiae,the Streptococcus uberis and the Streptococcus agalactiae were the following pathogenic bacterium with 8.97%,5.13% and 1.28%,respectively.We preliminary defined the situation of mastitis in Taizhou based on the results of this investigation,and this research would provide some scientific basis for further comprehensive prevention for dairy cows mastitis and develop mastitis drug therapy.