罗格列酮对糖尿病大鼠肾脏IL-6、IL-8、TNF-α表达的影响

Effects of Rosiglitazone on IL-6,IL-8 and TNF-α Expression in the Kidney of Diabetic Rats

目的观察罗格列酮对糖尿病大鼠肾脏炎症相关因子IL-6、IL-8和TNF-α表达的影响。方法雄性SD大鼠被分为正常组、正常+罗格列酮组、糖尿病组、糖尿病+罗格列酮组。所有大鼠均检测生化指标、尿白蛋白排泄率,及肾组织IL-6、IL-8、TNF-αmRNA和蛋白水平。结果罗格列酮干预的糖尿病大鼠和未干预组相比,肾组织IL-6、IL-8、TNF-α表达显著降低,尿白蛋白排泄减少,但血糖、肌酐无明显变化。结论罗格列酮可能通过抑制炎症相关因子的表达,从而降低尿白蛋白的排泄以保护肾脏,并且其作用独立于降糖之外。

Objective To observe the effects of Rosiglitazone on inflammatory cytokines expression in the kidney of diabetic rats.Methods Forty male SD rats were randomly divided into four groups：normal control group,normal control＋Rosiglitazone group,diabetes group,diabetes＋Rosiglitazone group.Diabetic nephropathy in rats was induced by intraperitoneal injection of streptozotocin（STZ,70 mg/kg）and the rats were killed after treatment for 8 weeks.Biochemical markers and urinary albumin excretion were measured.The expression levels of IL-6,IL-8 and TNF-α mRNA and protein were detected by real-time PCR and Western blot,respectively.Results As compared with untreated diabetic rats,Rosiglitazone significantly reduced the IL-6,IL-8 and TNF-α expression levels,and urinary albumin excretion in the kidney of diabetic rats.Meanwhile,there was no significant change in blood glucose and creatinine.Conclusion Rosiglitazone might improve renal function partly through inhibiting the inflammatory cytokine expression and reducing urinary albumin excretion in diabetic nephropathy.Furthermore,the protective effects of Rosiglitazone on the kidney were independent from its hypoglycemic effects.