5-氟尿嘧啶-壳聚糖微囊体外释放度测定方法的建立

Establishing a method for the determination of 5-fu-microcapsules release rate in vitro

目的:建立5-氟尿嘧啶-壳聚糖微囊体外释放度测定的方法。方法:通过紫外全波长扫描确定5-氟尿嘧啶最大吸收波长,以紫外分光光度法测定其含量,以直接释药法测定其体外释放度,同时进行了方法学考察。结果:5-氟尿嘧啶-壳聚糖微囊体外释药量在6～14 mg/L浓度范围内线性关系良好,平均回收率为100.69%,RSD为0.95%。结论:所建立的方法简单、方便、准确,适合于5-氟尿嘧啶-壳聚糖微囊体外释放度的测定。

Objective： To establish a detecting method for 5 fu microcapsules release rate in vitro.Methods： The longest absorb wave of 5 fu was scanned by ultraviolet whole wave length,its contents were determined by the ultraviolet spectrophotometry.The direct release method was used to test the microcapsules release degree in vitro,and examined by methodology.Results： The 5 fu microcapsules release rate in vitro had the better linear relation at the range of 6～14 mg/L.The average recovery was 100.69%,RSD was 0.95%.Conclusion： The method established is simple,convenient and accurate which is fitted for the determination of 5-fu-chitosan microcapsules release rate in vitro.