肺癌患者外周血CD_4^+CD_(25)^+CD_(127)-调节性T细胞水平变化及其临床意义

Changes of CD_4^+CD_(25)^+CD_(127)-regulatory T cells level and clinical significance in the peripheral blood of patients with lung cancer

目的通过检测肺癌患者外周血CD4+CD2+5CD127-调节性T(CD4+CD2+5CD127-Treg)细胞及相关免疫细胞水平和血浆IL-10、IFN-γ水平,探讨肺癌患者的细胞免疫功能异常及其临床意义。方法采用流式细胞仪(FCM)和酶联免疫吸附(ELISA)法分别检测45例原发性肺癌患者、14例肺部良性病变患者和20例健康者外周血中CD4+CD2+5CD127-Treg细胞及相关免疫细胞水平和血浆IL-10、IFN-γ水平。结果与肺部良性病变患者和健康者比较,肺癌患者外周血CD4+CD2+5CD127-Treg细胞水平及CD4+CD28-T细胞水平升高,CD4+、CD8+CD2+8-T细胞、CD5+6NK细胞水平及CD4+/CD8+值降低,血浆IL-10水平升高,IFN-γ水平降低。肺癌患者外周血CD4+CD2+5CD127-Treg细胞水平与血浆IL-10水平呈正相关,与血浆IFN-γ水平呈负相关。CD4+CD2+5CD127-Treg细胞、CD4+CD28-T细胞、CD5+6NK细胞水平及血浆IL-10、IFN-γ水平与临床分期有关。结论 CD4+CD2+5CD127-Treg细胞在肺癌发生、发展过程中可能起一定作用。

Objective To detect the levels of CD＋4CD＋25CD127-regulatory T（CD＋4CD＋25CD127-Treg） cells and the other related immunocyte subsets in the peripheral blood and plasma iterleukin-10（IL-10）,interferon-γ（IFN-γ） levels in patients with lung cancer,and to evaluate the dysfunction of cell immunity and its clinical significance.Methods The levels of CD＋4CD＋25CD127-Treg cells and the other related immunocyte subsets in the peripheral blood were detected by flow cytometry（FCM） method,and plasma IL-10 and IFN-γ levels were determined by enzyme linked immunosorbent assay（ELISA） kits in 45 patients with primary lung cancer,14 patients with benign lung disease and 20 healthy volunteers.Results The levels of CD＋4CD＋25CD127-Treg cells and CD＋4CD28-T cells in the peripheral blood of patients with lung cancer were significantly higher than those of patients with benign lung disease and healthy volunteers respectively.The levels of CD＋4,CD＋8CD＋28-T cells,CD＋56 NK cells and ratio of CD＋4/CD＋8 in the peripheral blood of patients with lung cancer were significantly lower than those in other groups.Plasma IL-10 level in patients with lung cancer was significantly higher than that in other groups.Plasma IFN-γ level in patients with lung cancer was significantly lower than that in other groups.The level of CD＋4CD＋25CD127-Treg cells in the peripheral blood was positively correlated with plasma IL-10 level and negatively correlated with plasma IFN-γ level in patients with lung cancer.The levels of CD＋8CD＋28,CD＋56 NK and CD＋4CD＋25CD127-Treg cells,plasma IL-10 and IFN-γ levels were correlated with clinical stage.Conclusion The levels of CD＋4CD＋25CD127-Treg cells in the peripheral blood might play an important role in the development,progression of lung cancer.