4种生物源化学诱抗物质对温室黄瓜幼苗盐胁迫逆境的诱抗效果比较

Comparison of the Induced-resistant Effect of Four Biological Substances on Greenhouse Cucumber Seedlings under Salt Stress

以黄瓜（Cucumis sativus L.）为材料,在温室生产环境条件下,采用根际施用结合叶面喷洒的方法,分别施用不同浓度水杨酸（Salicylic acid,SA）、油菜素内酯（Brassinolide,BR）、壳聚糖（Chitosan,Cts）、亚精胺（Spermdine,Spd）溶液,利用200 mmol.L-1NaCl进行盐胁迫,运用灰色关联度分析,研究了4种生物源化学诱抗物质对黄瓜幼苗盐胁迫逆境的诱抗作用以及在盐胁迫逆境条件下对黄瓜幼苗生长的影响,比较了不同生物源化学诱抗物质及其不同浓度的诱抗效果。结果表明,4种生物源化学诱抗物质对黄瓜幼苗盐胁迫都有较强的诱抗作用,都能促进盐胁迫逆境下黄瓜幼苗的正常生长;生物源化学诱抗物质具有明显的浓度效应,必须选用适宜的浓度才能收到良好的应用效果;综合评判结果以BR最优,且以BR为0.01 mg.L-1处理最好,适宜浓度范围为0.005~0.050 mg.L-1;SA对盐胁迫的诱抗效果相对较弱;Cts,Spd,SA最适宜的处理浓度分别是150,100,150 mg.L-1,适宜浓度范围分别为150~200,100~200,100~150 mg.L-1。

Different concentrations of salicylic acid（SA）,brassinolide（BR）,chitosan（Cts） and spermdine（Spd） were applied to the cucumber seedling by root injection and foliar spray.The effects of the four biological substances on cucumber seedling growth and inducing resistance were studied under salt stress of 200 mmol·L-1 NaCl by gray relationship analysis.The results showed that the four biological substances could increase the resistance of cucumber seedling to salt stress and could help seedling grow.The induced-resistant effects of biological substances showed dose-effect relationship,which meant that the induced-resistant effect was dependent on proper concentration.The comprehensive evaluation results showed that 0.01 mg·L-1 BR had the best effect,with the proper concentration range of BR of 0.005～0.05 mg·L-1,while the induced-resistant effect of SA is comparably weak.The optimal concentration of Cts,Spd and SA was 150 mg·L-1,100 mg·L-1,and 150 mg·L-1 respectively.The proper concentration range of Cts,Spd and SA was 150～200 mg·L-1,100～200 mg·L-1,and 100～150 mg·L-1 respectively.