蔬菜保护地土壤木霉菌的UP-PCR多样性

UP-PCR Diversity Analysis of Trichoderma Species in Soil Planted Vegetables in Greenhouse

为明确蔬菜保护地土壤中木霉菌的遗传多样性,采用通用引物PCR对蔬菜保护地部分土壤样品中分离到的木霉菌进行了多样性分析,并用UPMGA法对供试菌株的扩增片段数据进行图谱聚类分析。从13条通用引物中筛选出5条引物,分别为3-2,AS4,AS15 inv,AS19和L45;扩增出46条谱带,其中多态性条带43条,占总条带数的93.5%。当相似系数为0.74时,可将24个菌株划分为9个组,分别为A1,A2,B1,B2,B3,B4,B5,C1和C2。

Genetic diversity of 24 Trichoderma isolates,which were obtained from the different soil samples collected from some greenhouse vegetable fields of Hebei province,were analyzed by Universally Primed PCR.The results indicated that a total of 46 bands appeared after applification by using 5 primers,and 43 bands（93.5%） were polymorphic.24 Trichoderma isolates were classified into 9 groups at the similarity coefficiency of 0.74 by UP-PCR cluster analysis.It was concluded that UP-PCR could reflect the genetic relationship and difference of Trichoderma species,and could be used as an assistant method for Trichoderma classification.