小鼠免疫刺激后乳腺pIgR mRNA转录及乳特异性IgA含量的变化

Variation of pIgR Expression and Specific IgA Concentration in Mammary Gland of Mice after Immune Stimulus

为了探讨免疫刺激后昆明小白鼠乳腺pIgR基因mRNA转录和特异性IgA含量变化。选择48只健康受孕昆明小白鼠,分为A、B、C、D组,分别于分娩后第4天注射灭菌生理盐水、Lipase+灭菌生理盐水、空ISCOM和Lipase+LTB+ISCOM,每组注射12只,并于分娩后第8天和12天采集小鼠乳样、血样和乳腺组织,用间接ELISA法测定乳和血浆中的特异性IgA含量,用Real Time-PCR检测小鼠乳腺中pIgR基因的相对转录水平。结果表明,免疫刺激对小鼠血浆中特异性IgA的含量无显著性影响(P>0.05),但是免疫B组和D组中小鼠分娩后12 d的乳中特异性IgA含量显著高于8 d乳中的特异性IgA含量(P<0.05)。RealTime-PCR相对定量也表明,免疫B组和D组中小鼠分娩后12 d的乳腺pIgR基因转录水平显著高于8 d乳腺pIgR基因转录水平(P<0.05)。免疫刺激后小鼠乳腺pIgR基因转录水平的提高能增加乳中特异性IgA的含量。

To study the effect of immune stimulus on pIgR mRNA expression and specific IgA concentration in mammary gland.Forty eight KunMing white mice were divided into four groups,12 mice per group.They were injected different vaccines as following at days 4 after parturition,A group（CK） isotonic Na chloride,B group Lipase ＋ isotonic Na chloride,C group ISCOM,D group Lipase ＋ LTB ＋ ISCOM.The samples,including blood,milk and mammary gland issues,were collected at days 8 and days 12 after parturition.The specific IgA concentration in mouse plasma and milk was determined by indirect ELISA.The specific IgA concentration in mouse plasma was not significant among four groups（P 0.05）,whereas that in mouse milk of B and D group at days 12 was higher than that at days 8.And the results of RealTime-PCR also demonstrated that the pIgR gene expression in mammary gland at days 12 of group B and D was higher than that at days 8.Immune stimulus can enhance the expression of pIgR mRNA expression and specific IgA concentration,and the pIgR gene over-expression could increase the specific IgA level in milk.